Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDK8 (G398) Antibody #4101

Cell division protein kinase 8   cyclin   mediator complex   protein kinase k35   sc-1521  

No. Size Price
4101S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4101T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
4101 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 53 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CDK8 (G398) Antibody detects endogenous levels of total CDK8 protein. CDK8 (G398) Antibody 能够检测内源性CDK8总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly398 of human CDK8. Antibodies are purified using protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对CDK8蛋白甘氨酸398位的肽段免疫动物,利用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CDK8 (G398) Antibody. Western blot方法检测不同细胞提取物,使用的抗体为CDK8 (G398) Antibody。

Background

The mammalian Mediator Complex is a multi-subunit protein complex that couples specific transcriptional regulators to RNA polymerase II (Pol II) and the basal transcription machinery. Interactions between distinct Mediator subunits and transcription factors allow for specific gene regulation (reviewed in 1). 
 Mediator complex interactions control various biological processes including insulin signaling (2), NF-κB-dependent signaling (3), stem cell pluripotency and self renewal (4,5), and proliferation of colon cancer cells (6,7). 
 CDK8/Cyclin C, along with Med12 and Med13, constitutes a subcomplex within the Mediator Complex thought to act as a molecular switch, inhibiting Pol II recruitment and transcription initiation (8,9). Expression of CDK8 abrogates E2F-1-dependent inhibition of β-catenin activity in colon cancer cells (9). High levels of CDK8 coincide with high β-catenin-dependent transcription in colon cancer cells, and their proliferation can be inhibited by suppressing CDK8 expression (8). 哺乳动物中介体复合物是一个多亚基的蛋白复合物,它能够偶联特异性的转录调节子至RNA聚合酶II (Pol II)和基础的转录子装置。不同的中介体亚基和转录因子的相互作用允许特异性的基因调节(回顾1)。中介体复合物相互作用控制各种生物学过程,包括胰岛素信号(2),NF-κB-依赖的信号(3),干细胞多能性和自我更新(4,5)和结肠癌细胞增殖(6,7)。CDK8/Cyclin C,连同Med12和Med13,在中介体复合物内部组成亚复合体,可以充当分子开关的角色,抑制Pol II招募和转录起始(8,9)。CDK8的表达能够废除结肠癌细胞中E2F-1-依赖的β-连环蛋白的活性(9)。高水平的CDK8与结肠癌细胞中高水平的β-连环蛋白依赖的转录相一致,而且其增殖可以通过抑制CDK8的表达被抑制(8)。

  1. Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
  2. Wang, W. et al. (2009) Dev Cell 16, 764-71.
  3. van Essen, D. et al. (2009) PLoS Biol 7, e73.
  4. Tutter, A.V. et al. (2009) J Biol Chem 284, 3709-18.
  5. Varelas, X. et al. (2008) Nat Cell Biol 10, 837-48.
  6. Firestein, R. et al. (2008) Nature 455, 547-51.
  7. Morris, E.J. et al. (2008) Nature 455, 552-6.
  8. Knuesel, M.T. et al. (2009) Mol Cell Biol 29, 650-61.
  9. Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications