Cell Signaling Technology

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DRAQ5® #4084

cellular dyes   DNA dye   draq  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F
IF-F
IF-IC
IF-P

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IF-P=Immunofluorescence (Paraffin),

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 versus DRAQ5® (DNA content).流式细胞术分析Jurkat细胞,所用抗体为Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 对应 DRAQ5 ® (DNA content)。

IF-F

IF-F

Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody #2079 (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5™® (fluorescent DNA dye).激光共聚焦免疫荧光分析小鼠睾丸细胞,所用抗体为Miwi (G82) Antibody #2079 (绿色) 和 Pan-Keratin (C11) Mouse mAb #4545 (红色).蓝色伪彩为荧光DNA染料DRAQ5 ®

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞,所用抗体为COX IV (3E11) Rabbit mAb #4850 (绿色).肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料DRAQ5 ®

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).激光共聚焦免疫荧光分析石蜡包埋的人类结肠腺癌细胞,所用抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (绿色).蓝色伪彩为荧光DNA染料DRAQ5 ®。

Spectra

Spectra

Absorption and fluorescence emission spectra of DRAQ5® in aqueous solution.DRAQ5 ®在水溶性溶液中的吸收和荧光的发射光谱。

Directions for Use

Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection: 
 Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer. 
 Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

请参照CST公司建议的IF和Flow步骤,对于两种方法,请使用以下检测: 流式细胞术:离心细胞,用按照1:5000 (1 µM)将 DRAQ5 ® 加到0.5ml的孵育缓冲液中。避光室温孵育5分钟,然后采用流式细胞术分析细胞。 免疫荧光:用PBS清洗细胞2次,每次5分钟。按照1:1000(5uM)用PBS稀释e DRAQ5 ®,避光室温孵育5分钟,用PBS清洗细胞一次,盖上带有褪色试剂涂层盖玻片,采用适当的激发光波长尽快检测细胞。

Background

DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).

本产品是一种能透过细胞膜的远红DNA荧光染料,可以应用在肝脏中或用来固定细胞。这种染料可以与GFP或FITC标记结合。 DRAQ5 ® 在流式细胞术和荧光显微镜中检测细胞内的DNA(1-3)。

  1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
  2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
  3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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