Cell Signaling Technology

Product Pathways - Companion Products

DRAQ5® #4084

cellular dyes   DNA dye   draq  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IF-P=Immunofluorescence (Paraffin),

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 versus DRAQ5® (DNA content).流式细胞术分析Jurkat细胞,所用抗体为Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 对应 DRAQ5 ® (DNA content)。



Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody #2079 (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5™® (fluorescent DNA dye).激光共聚焦免疫荧光分析小鼠睾丸细胞,所用抗体为Miwi (G82) Antibody #2079 (绿色) 和 Pan-Keratin (C11) Mouse mAb #4545 (红色).蓝色伪彩为荧光DNA染料DRAQ5 ®



Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞,所用抗体为COX IV (3E11) Rabbit mAb #4850 (绿色).肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料DRAQ5 ®



Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).激光共聚焦免疫荧光分析石蜡包埋的人类结肠腺癌细胞,所用抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (绿色).蓝色伪彩为荧光DNA染料DRAQ5 ®。



Absorption and fluorescence emission spectra of DRAQ5® in aqueous solution.DRAQ5 ®在水溶性溶液中的吸收和荧光的发射光谱。

Directions for Use

Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection: 
 Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer. 
 Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

请参照CST公司建议的IF和Flow步骤,对于两种方法,请使用以下检测: 流式细胞术:离心细胞,用按照1:5000 (1 µM)将 DRAQ5 ® 加到0.5ml的孵育缓冲液中。避光室温孵育5分钟,然后采用流式细胞术分析细胞。 免疫荧光:用PBS清洗细胞2次,每次5分钟。按照1:1000(5uM)用PBS稀释e DRAQ5 ®,避光室温孵育5分钟,用PBS清洗细胞一次,盖上带有褪色试剂涂层盖玻片,采用适当的激发光波长尽快检测细胞。


DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).

本产品是一种能透过细胞膜的远红DNA荧光染料,可以应用在肝脏中或用来固定细胞。这种染料可以与GFP或FITC标记结合。 DRAQ5 ® 在流式细胞术和荧光显微镜中检测细胞内的DNA(1-3)。

  1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
  2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
  3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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