Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-Drosophila Akt (Ser505) Antibody #4054

akt1   akt2   akt3   Dakt   pkb  

No. Size Price
4054S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4054 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 D. melanogaster, Endogenous 65 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho Drosophila Akt (Ser505) Antibody detects endogenous levels of Akt only when phosphorylated at serine 505. It does not recognize drosophila Akt when phosphorylated at other sites, nor does it recognize related kinases such as PKC or p70 S6 Kinase.

Phospho Drosophila Akt (Ser505) Antibody能够检测内源性的Ser505位点磷酸化的Akt蛋白水平。该抗体不能够识别果蝇属Akt其他位点的磷酸化,以及其它相关激酶,如PKC和p70 S6激酶。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser505 of drosophila Akt. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与果蝇Akt蛋白的Ser505位点周围序列相对应的磷酸肽免疫动物生产而得到。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved (6 hours) S2 cells treated with 10 ug/ml insulin with or without 1 uM wortmannin pretreatment, using Phospho-Drosophila Akt (Ser505) Antibody (top) or Akt antibody #9272 (bottom).

Western blot 分析10 ug/ml胰岛素处理的血清饥饿(6小时)S2细胞提取物,并分别为应用渥曼青霉预处理或者未预处理组,使用的抗体为Phospho-Drosophila Akt (Ser505) Antibody(上图) 或者 Akt antibody #9272 (下图)。

Background

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).Drosophila Akt (D-Akt) regulates multiple biological processes such as cell survival (20) and cell growth (21). Major sites of phosphorylation include Ser505 and Thr342. These activation sites are homologous to mammalian Ser473 and Thr308 respectively. Identified downstream targets of D-Akt include Trh (Ser665) (22) and Tsc2 (23). Like Akt, D-Akt functions in a wortmannin-sensitive pathway involving PI3 kinase (24).

Akt,又被称为PKB 或 Rac,在细胞的生长和凋亡中起到关键作用(1-3)。该蛋白激酶可以被胰岛素和多种生长和存活因子激活,在涉及PI3K激酶的wortmannin敏感信号通路中发挥作用(2,3)。Akt可由磷脂结合激活,该过程通过活化环中的Thr308 (4)位点以及羧基端Ser473位点的磷酸化完成,其中Thr308的磷酸化由PDK1完成。 之前推测PDK2在Ser473位点磷酸化Akt,后被证实为哺乳动物rapamycin靶蛋白mTOR 的作用, 它存在在一个含有rictor和Sin1 的rapamycin非敏感复合体中。 Akt促进细胞的生长通过抑制细胞的凋亡 ,例如Akt可以抑制下游靶蛋白Bad (7), forkhead 转录因子 (8), c-Raf (9), and caspase-9。PTEN是PI3K/Akt信号通路的主要负调控因子(10)。 LY294002是特异性的 PI3K 激酶抑制剂(11)。Akt 的另外一个主要功能是通过磷酸化并进而抑制GSK-3α 和 β来调控糖原的合成(12,13)。 Akt 也可以调控胰岛素诱导的葡萄糖转运 (12)。除此之外,Akt还可以调控细胞周期,这个功能通过抑制GSK-3β,从而调控其下游的Cyclin D1 的磷酸化和降解 (14),或者负向调控cyclin依赖的激酶抑制因子 p27 Kip (15) 和 p21 Waf1/CIP1 (16)来实现。 Akt 也可以通过直接磷酸化含有raptor的rapamycin敏感复合体中的mTOR来调控细胞的生长(17)。 更重要的是, Akt磷酸化并失活TSC2, 而TCS2是mTOR-raptor复合物中mTOR的抑制因子(18,19)。果蝇属的Akt(D-Akt)调节多种生命活动如细胞存活和生长(21)。其主要的磷酸化位点包括Ser505和Thr342. 这些活化位点与哺乳动物中的Ser473和Thr308同源。已鉴定的D-Akt下游靶标包括Trh(Ser665)(22)和TSC2(23)。和Akt一样,D-Akt在包括PI3K激酶在内的对Wortmannin敏感的通路中其作用(24)。

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  2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
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  10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
  12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
  13. Cross, D.A. et al. (1995) Nature 378, 785-9.
  14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
  16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
  17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  20. Staveley, B. E. et al. (1998) Curr Biol 8(10) , 599-602.
  21. Scanga, S. E. et al. (2000) Oncogene 19, 3971-3977.
  22. Jin, J. et al. (2001) Dev Cell 1(6) , 726-728.
  23. Potter, C. J. et al. (2002) Nature Cell Biology 4, 658-665.
  24. Linassier, C. et al. (1997) Biochem. J. 321, 849-856.

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