Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Phospho-SMC1 (Ser360) Antibody #4029

chromosome cohesion   cohesion   DNA repair   Sb1.8   SMC   SMC1A   structural maintenance of chromosomes protein 1A  

No. Size Price
4029S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4029 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 145 Rabbit
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Monkey, Chicken, Xenopus, Bovine, S. cerevisiae,

Specificity / Sensitivity

Phospho-SMC1 (Ser360) Antibody detects endogenous levels of SMC1 protein only when phosphorylated on Ser360. This antibody does not cross-react with other SMC proteins. Phospho-SMC1 (Ser360) Antibody 能够检测内源性Ser360磷酸化的SMC1蛋白水平。该抗体不与其它的SMC蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser360 of the human SMC1 protein. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的针对人SMC1蛋白的360位丝氨酸的磷酸化肽段免疫动物生产的。抗体通过A蛋白和多肽亲和层析来纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or UV-treated (100 J/m2 followed by 4 hour recovery) using Phospho-SMC1 (Ser360) Antibody (upper) or SMC1 Antibody #4802 (lower). Antibody phospho-specificity was determined by treating cell extracts with λ phosphatase. Western blot 方法检测293细胞提取物,未处理或者紫外处理(100 J/m2 ,随后恢复4 hour),使用的抗体为Phospho-SMC1 (Ser360) Antibody (上) 或SMC1 Antibody #4802 (下)。抗体的磷酸化特异性通过使用λ 磷酸酶处理细胞来确定。



Confocal immunfluorescent analysis of HeLa cells, untreated (left) and UV-treated (right), using Phospho-SMC1 (Ser360) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 激光共聚焦荧光法检测HeLa细胞提取物,左图未处理,右图为紫外处理,使用的抗体为 Phospho-SMC1 (Ser360) Antibody (绿色)。肌动蛋白纤维使用DY-554 鬼笔环肽标记(红色)。


Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3). 染色体结构维持蛋白1(SMC1)是黏连蛋白复合体的一个染色体蛋白成员,该复合体促进姐妹染色单体结合并在DNA修复中起着重要作用(1,2)。作为S期内部DNA损伤检验点的一部分,ATM/NBS1-依赖的SMC1磷酸化发生在第957位和966位丝氨酸以应答离子辐射(IR) (3)。在经历其他形式的DNA损伤包括紫外线和羟基脲处理的细胞中,SMC1的磷酸化是ATM-非依赖的(4)。尽管SMC1的磷酸化是IR诱导的内S期检验点激活所必需的,但精确的机制尚未阐明且有可能涉及影响SMC1-SMC3相互作用的构象变化(3)。

The serine residue at 360 of SMC1 is phosphorylated in an ATM/ATR-dependent manner in response to DNA damage (5,6). Phospho-SMC1 (Ser360) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser360 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information. SMC1的第360位丝氨酸残基以ATM/ATR依赖性的方式被磷酸化以应答DNA损伤(5,6)。 通过PhosphoScan®和CST的LC-MS/MS修饰位点发现平台,Phospho-SMC1 (Ser360)抗体的作用位点已被鉴定。更多信息请访问PhosphoSitePlus®和修饰位点数据库(www.phosphosite.org)。

  1. Michaelis, C. et al. (1997) Cell 91, 35-45.
  2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
  3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
  4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.
  5. Stokes, M.P. et al. (2007) Proc Natl Acad Sci U S A 104, 19855-60.
  6. Matsuoka, S. et al. (2007) Science 316, 1160-6.

Application References

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