Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody #4027

amparnmdar   GPCR  

No. Size Price
4027S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4027 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 100 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody detects endogenous levels of GluR 2 only when phosphorylated at Tyr876. It may also detect GluR 3 when phosphorylated at the conserved Tyr887. This residue is not conserved in GluR 1 or GluR 4.

Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody 兔多抗识别内源性的Tyr876磷酸化的GluR 2蛋白。它也可以识别Tyr887磷酸化的GluR 3蛋白。这些残基在GluR 1或GluR 4中并不保守。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr876 of human GluR 2. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类GluR 2蛋白Tyr876位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody (upper) or AMPA Receptor (GluR 2/3/4) Antibody #2460 (lower).

Western blot分析大鼠大脑(假手术组、15分钟缺血后再灌注4小时或仅15分钟缺血),使用的抗体是Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody 兔多抗 (上图)或AMPA Receptor (GluR 2/3/4) Antibody 兔多抗#2460(下图)。

Background

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).Src family tyrosine kinases phosphorylate the GluR 2 subunit of AMPA receptors at Tyr876, which increases the interaction with GRIP1/2 but not PICK1. In addition, Tyr876 is important for AMPA- and NMDA-induced GluR 2 internalization (3). 
 The phosphorylation site at Tyr876 was also independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of GluR at Tyr876 was observed in extracts isolated from ischemic rat brain.

AMPA(α-氨基-3-羟基-5-甲基-4-恶唑酸),kainite和NMDA(N-甲基-D-天冬氨酸)受体是离子型谷氨酸门控离子通道的三个主要的家族。AMPA受体(AMPARs)是由四个亚基(GluR1-4)构成,以同源或异源四聚体的形式介导大多数中枢神经系统的兴奋性传递。AMPARs与突触的形成、稳定和可塑性有关(1)。缺乏GluR2的AMPARs可渗透钙,包含GluR2的AMPARs相反(2)。转录后修饰(剪接,RNA编辑核)和翻译后修饰(糖基化,磷酸化)可以导致结果的多样,可以微调AMPARs的动力学性质。AMPARs酶活性的变化与各种疾病有关,包括阿尔茨海默氏症,肌萎缩性侧索硬化症(ALS),中风,癫痫(1)。Src家族酪氨酸激酶磷酸化AMPA受体GluR 2亚基的Tyr876,从而增加GRIP1 / 2的相互作用,但不影响PICK1。此外,Tyr876对AMPA和NMDA诱导GluR 2内在化是重要的(3)。Tyr876磷酸化位点由CST公司使用PhosphoScan®和CST的磷酸化位点质谱平台发现。GluR2在Tyr876上的磷酸化在缺血大鼠脑组织分离提取物中可见。

  1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
  2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
  3. Hayashi, T. and Huganir, R.L. (2004) J. Neurosci. 24, 6152-6160.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications