Cell Signaling Technology

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Phospho-p130 Cas (Tyr249) Antibody #4014

No. Size Price
4014S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4014 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 130 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-p130 Cas (Tyr249) Antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 249. The antibody may cross-react with other phosphorylated tyrosines in the substrate domain of p130 Cas.

磷酸化的-p130 Cas(酪氨酸249)抗体仅仅在249位酪氨酸磷酸化时检测内源性p130 Cas的水平。该抗体可能与p130 Cas的底物结构域的其他磷酸化酪氨酸交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr249 of human p130Cas. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体由合成的磷酸化肽段免疫动物产生,该肽段对应人p130 Cas 的酪氨酸249周围残基,抗体由蛋白A和肽段亲和层析技术纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-p130Cas (Tyr249) Antibody (upper) or p130Cas antibody (lower).未处理或经过牛小肠碱性磷酸酶(CIP)处理的的NIH/3T3细胞提取物,使用Phospho-p130Cas (Tyr249) 抗体(上) 或 p130Cas 抗体 (下)进行western blot分析。

Background

p130 Cas (Crk-associated substrate) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) (1) and the FAK-related kinase PYK2 (2), as well as with the tyrosine phosphatases PTP-1B (3) and PTP-PEST (4). The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr668 and/or Tyr670) that can promote interaction with the SH2 domain of SFKs (5). The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr165, 249 and 410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains including adaptors, C-Crk, Nck and inositol 5'-phosphatase 2 (SHIP2) (6). The tyrosine phosphorylation of p130 Cas has been implicated as a key signaling step in integrin control of normal cellular behaviors including motility, proliferation and survival. Aberrant CAS tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins.

p130 Cas(Crk-相关底物) 是一个停泊蛋白,含有多个蛋白与蛋白相互作用结构域。氨基末端SH3可能起分子开关作用,调控CAS酪氨酸磷酸化,它与焦点粘附激酶(FAK)相互作用(1)也和FAK相关激酶PYK2(2)相互作用, 还和酪氨酸磷酸酶PTP-1B(3)和PTP-PEST(4)相互作用。C末端Src绑定结构域(SBD)含一个脯氨酸富集的基序,这个基序调节与Src家族激酶(SFKs)的SH3结构域以及一个酪氨酸磷酸化位点(酪氨酸668和/或酪氨酸670),这个酪氨酸位点可能促进与SFKs的SH2结构域相互作用(5)。p130 Cas中心底物结构域,酪氨酸磷酸化的主要区域,由15个酪氨酸存在于酪氨酸-X-X-脯氨酸(YXXP)基序,包括酪氨酸165、249和410.当被磷酸化时,大部分 YXXP基序能作为带有SH2或PTB结构域的蛋白的停泊位点,这些蛋白包括adaptors, C-Crk, Nck 和inositol 5'-磷酸酶 2 (SHIP2) (6)。p130 Cas的酪氨酸磷酸化在整合素调控正常细胞行为(迁移,增殖和存活)中牵涉关键信号通路步骤,不正常地CAS酪氨酸磷酸化可能通过特殊蛋白对细胞转化进行促进。

  1. Polte, T.R. and Hanks, S.K. (1997) J Biol Chem 272, 5501-9.
  2. Astier, A. et al. (1997) J Biol Chem 272, 228-32.
  3. Liu, F. et al. (1996) J Biol Chem 271, 31290-5.
  4. Garton, A.J. et al. (1997) Oncogene 15, 877-85.
  5. Ruest, P.J. et al. (2001) Mol Cell Biol 21, 7641-52.
  6. Bouton, A.H. et al. (2001) Oncogene 20, 6448-58.

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