Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Tuberin/TSC2 (D57A9) Rabbit mAb #3990

hamartin   sc-893  

No. Size Price
3990S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3990 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 200 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Tuberin/TSC2 (D57A9) Rabbit mAb detects endogenous levels of total tuberin protein.

Tuberin/TSC2 (D57A9) Rabbit mAb检测内源性的马铃薯球蛋白(Tuberin)的总含量。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human tuberin.

该单克隆抗体是采用与人类马铃薯蛋白(tuberin)羧基末端相对应的合成肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y and C6 cells, and from wild-type (wt) and TSC2-deficient (-/-) mouse embryonic fibroblasts (MEF), using Tuberin/TSC2 (D57A9) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western blot 分析SH-SY5Y 和C6 cells细胞提取物,来自于野生型(wt)和TSC2缺陷性(-/-)型小鼠的 胚胎fibroblasts (MEF),所使用的抗体是Tuberin/TSC2 (D57A9) Rabbit mAb兔单抗(上)和Akt (pan) (C67E7) Rabbit mAb兔单抗 #4691 (下) 。

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

马铃薯球蛋白(Tuberin)是TSC2肿瘤抑制基因的产物,是细胞增殖和肿瘤发展的重要调节因子(1)。TSC2或与之相关的TSC1(hamartin)的突变可以引起结节硬化复合体TSC(tuberous sclerosis complex),是一种常染色体显性遗传病,其特点为发生多种广泛的非恶性肿瘤(2)。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化(3)。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性(3-5)。另外,tuberin可以抑制mTOR(mammalian target of rapamycin), 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白(4E-BP1,一种转录起始抑制因子)的活性,并最终抑制翻译(3,6,7)。

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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