Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TRRAP (P2032) Antibody #3967

acetyltransferase   HAT  

No. Size Price
3967S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3967 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 434 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TRRAP (P2032) Antibody detects endogenous levels of total TRRAP protein.

TRRAP (P2032) Antibody能够检测内源性TRRAP总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human TRRAP protein. Antibodies are purified by peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of cell lysates from Jurkat, NIH/3T3 and COS cells using TRRAP (P2032) Antibody.

使用TRRAP (P2032) Antibody,免疫印迹(Western blot)分析Jurkat、NIH/3T3和COS细胞中TRRAP(P2032)的蛋白水平。


Transformation/transcription domain-associated protein (TRRAP) is a highly conserved 434 kDa protein found in various multiprotein complexes, such as SAGA, PCAF, NuA4 and TIP60, which contain histone acetyltransferase (HAT) activity (1-4). TRRAP functions as an adaptor protein by binding directly to the transactivation domains of transcriptional activator proteins and facilitating the recruitment of HAT complexes to acetylate histone proteins and activate transcription (1-5). TRRAP is required for the transcriptional activation and cell transformation activities of c-Myc, E2F1, E2F4, p53 and the adenovirus E1A proteins (1,6,7). TRRAP is also essential in early development and is required at the mitotic checkpoint and for normal cell cycle progression (8,9). In addition, TRRAP has been shown to function in DNA repair. As part of the TIP60 complex, TRRAP is required for the acetylation of histone H4 at double-stranded DNA breaks and subsequent DNA repair by homologous recombination (10). In addition, TRRAP associates with the MRN (MRE11, RAD50, NBS1) complex, which lacks intrinsic HAT activity yet functions in the sensing and subsequent repair of double-stranded breaks by non-homologous DNA end-joining (11). TRRAP shows significant homology to the PI-3 kinase domain of the ATM family of kinases; however, amino acids that map to the catalytic site of the kinase domain are not conserved in TRRAP (1).

Transformation/transcription domain-associated protein (TRRAP)是一个高度保守的434 kDa大小蛋白,该蛋白被发现在多种蛋白复合物中例如SAGA、PCAF、NuA4和TIP60,这些包含组蛋白乙酰转移酶(HAT)活性(1-4)。TRRAP功能作为一个接头蛋白,该蛋白通过直接结合到转录激活因子的转录激活区以及有助于HAT复合物的招募从而去使组蛋白乙酰化和激活转录(1-5)。TRRAP对于转录激活和c-Myc、E2F1、E2F4、p53以及腺病毒E1A蛋白的细胞转化激活是需要的(1,6,7)。TRRAP也是在早期发育期间有重要作用,并且在有丝分裂的检查点和正常细胞周期进程是需要的(8,9)。此外,研究显示TRRAP蛋白功能是在DNA修复起作用。作为TIP60复合物的部分,TRRAP对于在双链DNA中断和随后相应的重组的DNA修复中的histone H4的乙酰化是需要的(10)。此外,TRRAP与MRN (MRE11、RAD50、NBS1)复合物有关联,这缺少内部HAT活性,但是其功能在敏感性和随后双链中断的修复,该修复通过非同源DNA末端连接(11)。 TRRAP显示与ATM激酶家族的PI-3激酶区域有重要意义的同源性;然而,该激酶结构域的催化位点的氨基酸在TRRAP蛋白是不保守的(1)。

  1. McMahon, S.B. et al. (1998) Cell 94, 363-374.
  2. Grant, P.A. et al. (1998) Mol. Cell 2, 863-867.
  3. Ikura, T. et al. (2000) Cell 102, 463-473.
  4. Allard, S. et al. (1999) EMBO J. 18, 5108-5119.
  5. McMahon, S.B. et al. (2000) Mol. Cell. Biol. 20, 556-562.
  6. Deleu, L. et al. (2001) Oncogene 20, 8270-8275.
  7. Ard, P.G. et al. (2002) Mol. Cell. Biol. 22, 5650-5661.
  8. Herceg, Z. et al. (2001) Nat. Genet. 29, 206-211.
  9. Li, H. et al. (2004) EMBO J. 23, 4824-4834.
  10. Murr, R. et al. (2006) Nat. Cell Biol. 8, 91-99.
  11. Robert, F. et al. (2006) Mol. Cell. Biol. 26, 402-412.

Application References

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