Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) #3958

MAPKK-1   MAPKK-2   MEK   MEK-1   MEK-2  

No. Size Price
3958S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
3958 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 45 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) detects endogenous levels of MEK1/2 only when phosphorylated at Ser217/221.

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)兔单抗能够检测内源性Ser217/221位点磷酸化的MEK1/2蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2.



This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated antibody Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 reacts with human, mouse, rat, monkey and D. melanogaster phospho-MEK1/2 (Ser217/221). CST expects that Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) will also recognize phospho-MEK1/2 (Ser217/221) in these species.

Cell Signaling Technology的生物素偶联的抗体都是在优化条件下生产的。未偶联的Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154兔单抗可能会与人、大小鼠、猴以及黑腹果蝇的phospho-MEK1/2 (Ser217/221)发生反应。CST推测Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)也能识别这些物种中的phospho-MEK1/2 (Ser217/221)。

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Streptavidin-HRP (#3999).

western blot方法检测细胞提取物:未处理和TPA处理的HeLa细胞,使用的抗体是Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)兔单抗和 Streptavidin-HRP (#3999)。



Immunoprecipitation of extracts from HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Immobilized Streptavidin (Bead Conjugate) # 3419. Western blot analysis was performed using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154, Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 for detection.

免疫沉淀方法检测细胞提取物:未处理和TPA处理的HeLa细胞,使用的抗体是Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)兔单抗和Immobilized Streptavidin (Bead Conjugate) # 3419。western blot检测使用的抗体是Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154兔单抗, Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677和Anti-mouse IgG, HRP-linked Antibody #7076。


MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

MEK1、MEK2,也被称为MAPK或Erk激酶,它们是双特异性蛋白激酶,在丝裂原活化蛋白激酶级联反应中发挥作用,控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸(217 /221位点)的磷酸化而实现的,这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化,激活p44、 p42 MAPK激酶。

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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