Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

PHF20 (D96F6) XP® Rabbit mAb #3934

C20orf04   GLEA2   HCA58   NZF   PHD finger   TZP   zinc finger  

No. Size Price
3934S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
3934 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 150 Rabbit IgG
IP 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Bovine, Horse,

Specificity / Sensitivity

PHF20 (D96F6) XP® Rabbit mAb detects endogenous levels of total PHF20 protein.

PHF20 (D96F6) XP® Rabbit mAb兔单抗能够检测内源性PHF20总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the human PHF20 protein.

通过合成的与人源PHF20蛋白去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT116 and F9 cells using PHF20 (D96F6) XP® Rabbit mAb.

使用PHF20 (D96F6) XP® Rabbit mAb,免疫印迹(Western blot)分析HCT116和F9细胞中PHF20 (D96F6)的蛋白水平。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using PHF20 (D96F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

使用PHF20 (D96F6) XP® Rabbit mAb (绿色),共聚焦免疫荧光分析NTERA-2细胞。DY-554 phalloidin标记微丝蛋白(红色)。

Background

PHD finger protein 20 (PHF20) is a putative transcription factor protein. PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20 (1). PHF20 may contribute to the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer (2-5).

PHD finger protein 20 (PHF20)是一个转录因子。PHF20包含一个tudor结构域,这有助于结合到双甲基化的histone H4蛋白Lys20位点(1)。PHF20可能有助于癌症的发展,包括恶性胶质瘤、肺癌、结肠癌和卵巢癌(2-5)。

  1. Kim, J. et al. (2006) EMBO Rep 7, 397-403.
  2. Fischer, U. et al. (2001) Clin Exp Immunol 126, 206-13.
  3. Taniwaki, M. et al. (2006) Int J Oncol 29, 567-75.
  4. Bankovic, J. et al. (2009) Lung Cancer 67, 151-9 .
  5. Wang, Y. et al. (2002) J Immunol 169, 1102-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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