Cell Signaling Technology

Product Pathways - Protein Stability

Ubiquitin Antibody #3933

sc-8017   ub   UBA52   UBA80   UBB   UBC   UBCEP1   UBCEP2  

No. Size Price
3933S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3933 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit
IHC-P 1:600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: D. melanogaster, Xenopus, Zebrafish, Bovine, Pig, Horse,

Specificity / Sensitivity

Ubiquitin抗体识别单泛素、多泛素和泛素化蛋白,这一抗体可能会与重组NEDD8发生交叉反应。

Source / Purification

此抗体是通过合成对应于人泛素蛋白氨基端序列的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 90 minutes), using Ubiquitin Antibody. 使用泛素抗体对未经或经26S蛋白酶体抑制剂MG132处理(10 µM,90分钟)的HeLa和NIH/3T3细胞裂解物的免疫印迹分析结果。 Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ubiquitin Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 在存在对照肽(左)或特异性抗原肽(右)的石蜡包埋的人乳腺癌标本中使用泛素抗体进行免疫组化分析的结果。 Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Ubiquitin Antibody. 在石蜡包埋人肺癌标本使用泛素抗体进行免疫组化分析的结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 90 minutes), using Ubiquitin Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ubiquitin Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Ubiquitin Antibody.

Background

泛素是一个在泛素——蛋白酶体途径中发挥重要作用的保守多肽。泛素能够通过泛素转移过程被共价修饰于多个细胞蛋白,导致蛋白通过26S蛋白酶体降解。三个泛素化酶参与泛素偶联过程:泛素首先与活化酶E1形成硫酯键复合体而被激活,然后被转移到泛素载体蛋白E2,然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3)。泛素——蛋白酶体途径参与多种生物过程和疾病发生,多种信号通路蛋白如IκB、p53、cdc25a 和Bcl-2都受到泛素——蛋白酶体途径调节,影响细胞周期进程、分化、细胞应激和凋亡等过程(4-7)。

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
  5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
  6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
  7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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