Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) #3929

4E-BP1   4ebp   BP1   PHAS-1  

No. Size Price
3929S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
3929 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 15 to 20 Rabbit IgG
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites.

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗检测仅在Thr37或Thr46位点磷酸化的内源性4E-BP1蛋白水平。该抗体可以与在等效位点磷酸化的4E-BP2和4E-BP3蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.

通过人工合成小鼠4E-BP1 蛋白Thr37和Thr46位点周围序列相应的多肽片段去免疫动物从而制备单克隆抗体。

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855.

此Cell Signaling Technology公司的抗体在最优条件接合生物素。这个抗体与未接合的Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗 #2855具有同样的物种的交叉反应活性。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved and stimulated with insulin (100 nM for 5 minutes), with (+) or without (-) λ phosphatase treatment, using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (upper) and 4E-BP1 (53H11) Rabbit mAb #9644 (lower).

使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (上图)和4E-BP1 (53H11) Rabbit mAb兔单抗 #9644 (下图),Western Blot分析在HeLa细胞系中磷酸化4E-BP1和4E-BP1蛋白的水平。细胞在无血清饥饿培养后再用胰岛素刺激(100 nM,5分钟),然后分为λ磷酸酶处理组(+)和未处理组。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb (Biotinylated).

使用Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb兔单抗 (Biotinylated),流式细胞术分析Jurkat细胞,细胞分为未处理组(绿色)和LY294002, Wortmannin 和U0126处理组(蓝色)。

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

翻译抑制蛋白4E-BP1 (又称 PHAS-1)通过结合翻译起始因子eIF4E蛋白从而抑制帽子结构依赖的翻译。4E-BP1的过磷酸化干扰上述结合并且导致激活帽子结构依赖的翻译(1)。PI3 kinase/Akt通路和FRAP/mTOR激酶通路都调节4E-BP1蛋白的激活(2,3)。在体内4E-BP1多个位点残基被磷酸化(4)。通过FRAP/mTOR蛋白使4E-BP1在Thr37和Thr46位点磷酸化不能阻止它eIF4E结合,但这被认为是为随后在4E-BP1的Ser65和Thr70位点磷酸化进行准备(5)。

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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