Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-PSD95 (Tyr236/Tyr240) Antibody #3919

No. Size Price
3919S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3919 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 95 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-PSD95 (Tyr236/Tyr240) Antibody detects endogenous levels of PSD95 protein only when phosphorylated at Tyr236 or Tyr240.

Phospho-PSD95 (Tyr236/Tyr240) Antibody 兔多抗识别内源性的Tyr236和Tyr240磷酸化的PSD95蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr236 and Tyr240 of human PSD95. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类PSD95蛋白Tyr236和Tyr240位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either sham operated or subjected to ischemia (15 min) alone or followed by reperfusion (4 hours), using Phospho-PSD95 (Tyr236/Tyr240) Antibody (upper) and PSD95 Antibody #2507 (lower).

Western blot分析大鼠大脑(假手术、仅15分钟缺血或15分钟缺血后再灌注4小时),使用的抗体是Phospho-PSD95 (Tyr236/Tyr240) Antibody 兔多抗(上图)或 PSD95 Antibody 兔多抗#2507(下图)。

Background

Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, a SH3 domain and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).Phospho-PSD95 (Tyr236/Tyr240) Antibody is directed against previously unpublished PSD95 tyrosine phosphorylation sites at Tyr236 and Tyr240 that were identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PSD95 at Tyr236 and Tyr240 was observed in extracts isolated from ischemic rat brain. The sites were independently found in a large-scale identification of tyrosine phosphorylation sites from murine brain (6).

突触后密集区蛋白95 (Postsynaptic Density protein 95,PSD95) 是一种膜相关鸟苷酸激酶(PSD - MAGUK)蛋白质PSD亚族的成员。该家族成员由三个PDZ结构域后的可变N末端,一个SH3结构域和一个无效的鸟苷酸激酶(GK)结构域组成。PSD95是一种脚手架蛋白,参与突触后密集复合物的装配和功能。(1,2)。PSD95通过间接方式参与AMPA受体在突触的定位,涉及Stargazin和相关的跨膜AMPA受体调节蛋白(TARPS)(3)。PSD95与经验依赖的可塑性有关,在学习中起着不可或缺的作用(4)。PSD95基因突变与自闭症有关(5)。Phospho-PSD95 (Tyr236/Tyr240) Antibody 兔多抗用来直接识别前述未发表的PSD95络氨酸磷酸化位点Tyr236和Tyr240。它们由CST公司使用PhosphoScan®和CST的磷酸化位点质谱平台发现。PSD95在Tyr236和Tyr240的磷酸化在缺血大鼠脑组织分离提取物中可见。这些位点从小鼠大脑的酪氨酸磷酸化大规模鉴定中被独立发现(6)。

  1. Cao, J. et al. (2005) J. Cell Biol 168, 117-26.
  2. Chetkovich, D.M. et al. (2002) J. Neurosci. 22, 6415-25.
  3. Cai, C. et al. (2006) J. Biol. Chem. 281, 4267-73.
  4. Yao, W.D. et al. (2004) Neuron 41, 625-38.
  5. Cline, H. (2005) Curr. Biol. 15, R203-5.
  6. Ballif, B.A. et al. (2008) J. Proteome Res. 7, 311-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications