Cell Signaling Technology

Product Pathways - Metabolism

Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb #3918


No. Size Price
3918S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3918T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3918 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 95 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-IGF-1 Receptor beta (Tyr1135) (DA7A8) Rabbit mAb detects endogenous levels of IGF-I receptor only when phosphorylated at Tyr1135. This antibody cross-reacts with Tyr1150 of insulin receptor and may also cross-react with other overexpressed related tyrosine-phosphorylated tyrosine kinases.

Phospho-IGF-1 Receptor beta (Tyr1135) (DA7A8) Rabbit mAb用于检测内源性在Tyr1135上磷酸化的IGF-1 Receptor beta,本抗体会与胰岛素受体Tyr1150交互反应,同时也可能与过表达的相关酪氨酸磷酸化的酪氨酸激酶交互反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1135 of human IGF-I receptor beta.

单克隆抗体通过用对应于人源IGF-1 Receptor beta蛋白Tyr1135序列的合成肽免疫动物制备而得

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated or stimulated with IGF-I, using Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb (upper) and IGF-I Receptor β Antibody #3027 (lower).


Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

一型胰岛素样生长因子受体(IGF-IR)是一种跨膜的酪氨酸激酶,在许多新生及成熟的组织细胞中都广泛表达(1-3)。IGF-I和IGF-II配体结合到受体上去,受体就发生自我磷酸化。在激酶结构域,有三个酪氨酸残基(Tyr1131, Tyr1135,Tyr1136)是最先发生自身磷酸化的位点(4)。这三个残基的磷酸化对激酶的激活是必需的(5,6)。胰岛素受体(IRs)跟胰岛素样生长因子受体有着相似的结构和功能,在它的激酶活化环上同样有三个酪氨酸簇(Tyr1146/1150/1151)。胰岛素受体酪氨酸残基的磷酸化是细胞接受胰岛素刺激后最先发生的生化反应(7)。自身磷酸化最先在Tyr1146和Tyr1150(或Tyr1151)发生,但激酶的激活需要三者同时磷酸化(8)。

  1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
  2. Baserga, R. (2000) Oncogene 19, 5574-81.
  3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
  4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
  5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
  6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
  7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
  8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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