Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Bcr (Tyr177) Antibody #3901

Abl   BCR-Abl  

No. Size Price
3901S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3901 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 160 (Bcr); 210 (Bcr-Abl) Rabbit
IHC-P 1:50
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Bcr (Tyr177) Antibody detects endogenous levels of Bcr and Bcr-Abl only when phosphorylated at tyrosine 177.

Phospho-Bcr (Tyr177)兔多抗能检测酪氨酸(177位点)磷酸化的内源性Bcr、Bcr-Abl蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr177 of human Bcr. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体通过使用与人源Bcr蛋白酪氨酸(177位点)周围残基相一致的磷酸化的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-Bcr (Tyr177) Antibody (upper) or Bcr Antibody #3902 (lower).western blot方法检测细胞提取物:未经处理和牛小肠磷酸酶CIP)处理的 K562细胞,使用的抗体是Phospho-Bcr (Tyr177) Antibody (上图) 和 Bcr Antibody #3902 (下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded bone marrow from a patient with chronic myelogenous leukemia, showing membrane and cytoplasmic localization, using Phospho-Bcr (Tyr177) Antibody.免疫组织化学方法检测石蜡包埋的白血病病人骨髓组织,显示胞膜和细胞质位置。使用的抗体是Phospho-Bcr (Tyr177) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from PAE/CKR cells (expressing chimeric receptors of the extracellular domain of CSF-1R, and transmembrane and cytoplasmic domains of KDR) stimulated with CSF-1 (40 ng/ml for 5 minutes) or NIH/3T3 cells stimulated with PDGF (40 ng/ml for 2 minutes) using Phospho-Bcr (Tyr177) Antibody (upper) or Bcr Antibody #3902 (lower).western blot方法检测细胞提取物:CSF-1 (40 ng/ml,5 min)刺激的PAE/CKR细胞(表达CSF-1R胞外区域的嵌合受体)和NIH/3T3细胞刺激的PDGF (40 ng/ml,2 min)。使用的抗体是Phospho-Bcr (Tyr177) Antibody (上图) 和 Bcr Antibody #3902 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, untreated (green) or Gleevec® (STI571)- treated (blue), using Phospho-Bcr (Tyr177) Antibody compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物:未经处理(绿色)的和Gleevec® (STI571)处理的(蓝色)K562细胞,使用的抗体是Phospho-Bcr (Tyr177),红色表示非特异性阴性对照抗体。

Background

The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac (2,3). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).

Bcr蛋白基因最早在嵌合的 Bcr-Abl致癌基因中发现(1),Bcr蛋白胺端区域包含一个寡聚化反应区域——丝氨酸/苏氨酸激酶区域,以及能结合SH2的区域。该蛋白质中心区域有一个PH结构域和一个与鸟苷酸交换因子序列相近的区域,是Rho家族的GTP结合蛋白。C端区域可能涉及到一个对小GTP结合蛋白Rac 的GTP酶活化作用(2,3)。原株Bcr in细胞的作用机制尚不清楚。PDGF受体可能将Bcr蛋白当作下游信号调节子来使用(4)。研究表明Bcr-Abl的融合导致结构性活化酪氨酸激酶的产生,该激酶会引起白血病(CML)(5). Bcr-Abl融合蛋白中Bcr蛋白酪氨酸(177位点)被磷酸化,该磷酸化作用对转变Bcr-Abl蛋白的活性十分重要(6)。磷酸化的酪氨酸(177位点)为Gab2 、GRB2提供了一个“停泊位点”(7,8)。

  1. Groffen, J. et al. (1984) Cell 36, 93-99.
  2. Maru, Y. et al. (1991) Cell 67, 459-468.
  3. Che, W. et al. (2001) Circulation 104, 1399-1406.
  4. Abe, J. I. et al. (2001) Ann. N.Y. Acad. Sci. 947, 341-343.
  5. Voncken, J. W. et al. (1995) Cell 80, 719-728.
  6. He, Y. et al. (2002) Blood 99, 2957-2968.
  7. Sattler, M. et al. (2002) Cancer Cell 1, 479-492.
  8. Warmuth, M. et al. (1995) J. Biol. Chem. 272, 33260-33270.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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