Cell Signaling Technology

Product Pathways - Metabolism

Phospho-Glycogen Synthase (Ser641) Antibody #3891

GS   GSY   GSY-1   Gys  

No. Size Price
3891S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3891 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 85 to 90 Rabbit
IP 1:100
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: Horse,

Specificity / Sensitivity

Phospho-Glycogen Synthase (Ser641) Antibody detects endogenous levels of both muscle and liver isoforms of glycogen synthase only when phosphorylated at serine 640 or 641 of the muscle and liver isoforms, respectively.

Phospho-Glycogen Synthase (Ser641) Antibody可同时检测内源性肌肉和肝脏的在640位或641位磷酸化的糖元合成酶。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser641 of human liver glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是通过合成对应于人肝脏GS蛋白Ser641序列的肽段免疫动物获得,抗体经Protein A和肽亲和层析纯化制得。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells treated with calf intestinal alkaline phosphatase (CIP), using Phospho-Glycogen Synthase (Ser641) Antibody (upper) or control glycogen synthase antibody (lower).

Wetern blot检测NIH/3T3细胞提取物,细胞用calf intestinal alkaline phosphatase (CIP)处理,所用抗体为Phospho-Glycogen Synthase (Ser641) Antibody (上)和control glycogen synthase antibody (下)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Glycogen Synthase (Ser641) Antibody.

免疫组织化学检测石蜡包埋的人乳腺癌组织,所用抗体为Phospho-Glycogen Synthase (Ser641) Antibody,显示胞质定位

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded renal clear cell adenocarcinoma using Phospho-Glycogen Synthase (Ser641) Antibody.

免疫组织化学检测石蜡包埋的人肾透明细胞腺瘤组织,所用抗体为Phospho-Glycogen Synthase (Ser641) Antibody

Background

Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).

糖元合成酶(GS)是糖元合成调节中的关键酶。哺乳动物有两种GS同工酶:肌肉GS和肝脏GS(1-2)。肌肉GS在多个组织中都有表达,而肝脏GS似乎是组织特异性的(3)。GS受到多个位点磷酸化的调控,同时也受变构配体结合的调控。磷酸化会抑制GS活性,但活性可被变异配体6-磷酸葡萄糖结合后恢复,6-磷酸葡萄糖可以诱导GS的去磷酸化从而激活。

Ser641 is one of the most important phosphorylation sites for the regulation of glycogen synthase (5).

Ser641是糖元合成酶最重要的磷酸化调控位点。

  1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
  2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
  3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
  4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
  5. Skurat, A.V. and Roach, P.J. (1995) J. Biol. Chem. 270, 12491-12497.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications