Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Gab2 (Ser159) Antibody #3884

GRB-associated-binder   GRB-associated-binder-2   Grb2-associated-binder   GRB2-associated-binding-protein-2  

No. Size Price
3884S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3884 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 98 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Gab2 (Ser159) Antibody detects endogenous levels of Gab2 only when phosphorylated at serine 159. This antibody does not cross-react with Gab1 or Gab3.

Phospho-Gab2 (Ser159)能够检测Ser159位点磷酸化的内源性Gab2蛋白水平。该抗体不和Gab1和Gab3发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 of human Gab2. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的与人源Gab2蛋白Ser159位点周围序列相对应的的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from H-4-II-E cells, untreated, HGF-treated, insulin-treated or treated with lambda phosphatase after lysis, using Phospho-GAB2 (Ser159) Antibody.

Western blot 检测分析H-4-II-E细胞提取物,分为未处理组, HGF处理组,胰岛素处理组或者γ-phosphatase处理组,使用的抗体是 Phospho-GAB2 (Ser159) 。

Background

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).Akt phosphorylates Gab2 at Ser159. This phosphorylation inhibits Gab2 tyrosine phosphorylation and downstream signal amplification (10).

Grb相关结合因子(Gab)家族是一类由多种受体酪氨酸激酶(RTKs),如EGFR,HGFR,胰岛素受体,细胞因子受体和B细胞抗原受体招募的接头蛋白。RTKs受到它们的同源配体刺激后,Gab被招募到细胞膜上,接着其被磷酸化,并起到支架的作用(1-4)。Gab1蛋白存在多个酪氨酸磷酸化位点(5)。酪氨酸(472位点)磷酸化可以调节其与PI3K激酶p85的结合(6,7)。Gab1酪氨酸(307,373和407位点)的磷酸化可以调节Gab1与PLCγ的互作(8)。酪氨酸(627和659位点)磷酸化是Gab1结合并活化蛋白酪氨酸磷酸酶SHP2所必需的(6,9)。Akt在Ser159位磷酸化Gab2。这个磷酸化抑制Gab2酪氨酸磷酸化以及信号向下游的扩大化。

  1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
  2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
  3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
  4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
  5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
  6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
  7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
  8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
  9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.
  10. Lynch, D.K. and Daly, R.J. (2002) EMBO J 21, 72-82.

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