Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-FRA1 (Ser265) Antibody #3880

fos   fos-related antigen   fosl1   fra-1   fra1   sc-605  

No. Size Price
3880S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3880 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 40 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Horse,

Specificity / Sensitivity

Phospho-FRA1 (Ser265) Antibody detects endogenous levels of FRA1 protein only when phosphorylated on Ser265. This antibody also shows minor cross-reactivity with phospho-FRA2 and phospho-c-Fos, but does not cross-react with phospho-FosB.

Phospho-FRA1 (Ser265) Antibodyt兔多抗能检测内源性丝Ser265位点磷酸化的FRA1蛋白水平。该抗体也会与phospho-FRA2、phospho-c-Fos 蛋白发生微交叉反应,但是不与phospho-FosB蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser265 of the human FRA1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体通过使用与人源 FRA1 蛋白Ser265位点周围残基相一致的磷酸化的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and TPA-stimulated for 4 hours, using Phospho-FRA1 (Ser265) Antibody #3880 (upper) and β-Actin Antibody #4967 (lower). Antibody phospho-specificity is shown by treating lysates with λ phosphatase.Western blot方法检测细胞提取物:无血清培养过夜的和TPA刺激四小时后的的HeLa细胞,使用的抗体是Phospho-FRA1 (Ser265) Antibody #3880 (上图)和 β-Actin Antibody #4967 (下图).。

Background

The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).

细胞核原癌基因的Fos家族成员包括c-Fos、FosB、Fos-相关抗原1 (FRA1)、Fos-相关抗原2 (FRA2)。虽然大部分Fos蛋白以单一亚型存在,但是FosB蛋白以两种亚型存在:全长的FosB蛋白和缩短的形式——FosB2 (Delta FosB),后者缺乏羧端的101个氨基酸(1-3)。Fos蛋白的表达是由细胞外刺激子快速且瞬间诱导的,这些刺激因子包括生长因子、细胞因子、神经传导物质、多肽激素和应激反应。Fos蛋白和Jun蛋白(c-Jun, JunB, 和JunD)结合成二聚体形成活化因子蛋白-1 (AP-1),它是结合到TRE/AP-1元件上的转录因子,并且能激活转录。Fos、Jun蛋白包含一个亮氨酸拉链基序,能调节二聚化和结合DNA的一个毗邻碱性域。各种Fos/Jun二聚体的不同点在于反式激活AP-1依赖性基因的能力。Fos蛋白对细胞外刺激因子应答后,除了表达量增加,同时也被Erk蛋白磷酸化,它的磷酸化可能会进一步增强转录活性(4-6)。Erk5蛋白引起的c-Fos蛋白Ser32位点和Thr232位点的磷酸化增强了蛋白质稳定性和核定位(5)。Erk1/2引起的FRA1蛋白Ser252、Ser265位点的磷酸化增强了蛋白稳定性,导致癌细胞内FRA1蛋白的过量表达(6)。生长因子刺激后,相对静止的成纤维细胞中FosB、c-Fos蛋白表达在短时间内被激活,但是几个小时后就消失了(7)。FRA1、 FRA2的表达持续时间更长,而且在异步生长细胞中能检测到它(8)。c-Fos, FosB, 和FRA2的失控表达将导致细胞的癌变;然而Delta FosB蛋白能阻止这种细胞转化的能力(2,3)。

  1. Tulchinsky, E. (2000) Histol Histopathol 15, 921-8.
  2. Dobrazanski, P. et al. (1991) Mol Cell Biol 11, 5470-8.
  3. Nakabeppu, Y. and Nathans, D. (1991) Cell 64, 751-9.
  4. Rosenberger, S.F. et al. (1999) J Biol Chem 274, 1124-30.
  5. Sasaki, T. et al. (2006) Mol Cell 24, 63-75.
  6. Basbous, J. et al. (2007) Mol Cell Biol 27, 3936-50.
  7. Kovary, K. and Bravo, R. (1991) Mol Cell Biol 11, 2451-9.
  8. Kovary, K. and Bravo, R. (1992) Mol Cell Biol 12, 5015-23.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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