Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (DM1A) Mouse mAb #3873

a-tubulin   sc-5286   sc-5546   sc-58666   sc-8035  

No. Size Price
3873S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3873T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
3873 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 52 Mouse IgG1
IHC-P 1:50
IF-IC 1:4000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

α-Tubulin (DM1A) Mouse mAb detects endogenous levels of total α-tubulin protein.

α-Tubulin (DM1A) Mouse mAb检测内源性α-tubulin总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length chicken α-tubulin purified from brain extracts, and recognizes residues surrounding Val440 of α-tubulin protein.

通过来自大脑提取物的全长鸡纯化的α-tubulin蛋白去免疫动物从而制备出单克隆抗体,并且识别α-tubulin蛋白Val440位点残基。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and COS-7 cells using α-Tubulin (DM1A) Mouse mAb.

使用α-Tubulin (DM1A) Mouse mAb,免疫印迹(Western Blot)分析HeLa和COS-7细胞中α-Tubulin蛋白水平。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Tubulin (DM1A) Mouse mAb.

使用α-Tubulin (DM1A) Mouse mAb,免疫组化分析人源结肠癌组织石蜡切片。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb (green). Red = Propidium Iodide (fluorescent DNA dye).

使用α-Tubulin (DM1A) Mouse mAb (绿色),共聚焦免疫荧光观察HeLa细胞。红色= Propidium Iodide (DNA荧光染料)。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications