Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FRS2-α (Tyr436) Antibody #3861

rtkscaffold   SNT  

No. Size Price
3861S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3861 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 80 to 85 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-FRS2-alpha (Tyr436) Antibody detects endogenous levels of FRS2-alpha only when phosphorylated at tyrosine 436. The antibody does not cross-react with unrelated tyrosine-phosphorylated proteins.

Phospho-FRS2-alpha (Tyr436) Antibody兔多抗能检测内源性酪氨酸(436位点)磷酸化的FRS2-α蛋白水平。该抗体不与无关的酪氨酸磷酸化蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr436 of human FRS2-alpha. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells cotransfected with human FRS2-alpha and rat TrkA, untreated or NGF-stimulated, using Phospho-FRS2-alpha (Tyr436) Antibody. (Cell lysates provided by Dr. Susan Meakin, Robarts Research Institute, London, Ontario.)Western blot方法检测细胞提取物:未经处理的和NGF刺激的转染人FRS2-alpha 和大鼠 TrkA的COS细胞,使用的抗体是Phospho-FRS2-alpha (Tyr436) Antibody. (细胞裂解液由Dr. Susan Meakin提供, 罗巴茨研究院, 伦敦, 安大略湖.)


Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). Activation of the FGFR leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-alpha (SNT1) and FRS2-beta (SNT2) (2), which are phosphorylated by these RTKs. Once they are phosphorylated, they recruit SH2 domain-containing proteins including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6).

成纤维细胞生长因子受体底物 (FRS2,也被称为Suc-相关的神经营养因子诱导的酪氨酸磷酸化靶点或SNT)参与将信号从成纤维细胞生长因子受体 (FGFR)向细胞外传送。FGFR的活化导致FRS2的酪氨酸磷酸化 (1)。目前已鉴定出两个FRS2家族成员,FRS2-alpha (SNT1) 和 FRS2-beta (SNT2) (2),这两种蛋白都被这些RTKs磷酸化。一旦这些蛋白被磷酸化,它们就会聚集含有SH2结构域的蛋白,包括Grb2 and SHP-2 (3,4),以此调节下游信号传递。酪氨酸(436位点)是SHP-2能有效聚集所必需的(5),然而酪氨酸(196位点)的作用是抑制Grb2-Sos复合物(6)。

  1. Kouhara, H. et al. (1997) Cell 89, 693-702.
  2. Ong, S. H. et al. (2000) Mol. Cell. Biol. 20, 979-989.
  3. Kontaridis, M. I. et al. (2002) Mol. Cell. Biol. 22, 3875-3891.
  4. Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276, 13049-13056.
  5. Hadari, Y. R. et al. (1998) Mol. Cell. Biol. 18, 3966-3973.

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