Cell Signaling Technology

Product Pathways - Protein Stability

UBE2L3 Antibody #3848

E2-F1   E2conjugating   L-UBC   UBCE7   UBCH7  

No. Size Price
3848S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3848 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 17 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

UBE2L3抗体识别内源性的UBE2L3总蛋白。

Source / Purification

UBE2L3多克隆抗体是通过合成与人UBE2L3蛋白Lys145邻近的氨基酸残基序列一致的肽段,免疫动物获得,抗体经肽亲和层析纯化。 Western blot analysis of extracts from various cell types using UBE2L3 Antibody. 使用UBE2L3抗体对各种类型细胞提取物中该蛋白进行检测的Western blot分析结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using UBE2L3 Antibody.

Background

蛋白泛素化作用需要E1, E2, 和E3泛素-结合酶的协同作用。泛素最先通过具有硫酯的泛素激活酶E1通过ATP-依赖形式活化。活化的泛素然后通过含有巯基的泛素载体蛋白E2转运。最后从E2的泛素转运到含有ε-氨基的赖氨酸残基靶蛋白,这由泛素连接酶E3介导(1)。

UBCH7(也称UBE2L3)是一个17kDa的泛素E2共价结合酶 (3)。它能与不同的E3泛素连接酶相互作用,包括E6-AP、SRC-1、c-Cbl、parkin等蛋白,调节多条信号通路并与帕金森发病有关(4-7)。

  1. Hershko, A. (1988) J Biol Chem 263, 15237-40.
  2. Rajendra, R. et al. (2004) J. Biol. Chem. 279, 36440-36444.
  3. Nuber, U. et al. (1996) J. Biol. Chem. 271, 2795-2800.
  4. Verma, S. et al. (2004) Mol. Cell Biol. 24, 8716-8726.
  5. Garside, H. et al. (2006) J. Endocrinol. 190, 621-629.
  6. Shimura, H. et al. (2001) Science 293, 263-269.
  7. Simmons, A. et al. (2005) Immunity 23, 621-634.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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