Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-AP2M1 (Thr156) Antibody #3843

A2M1   Adaptin-mu2   AP-2 mu-2 chain   AP2M1   CLAPM1   mu-2  

No. Size Price
3843S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3843 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-AP2M1 (Thr156) Antibody detects endogenous levels of AP2M1 protein only when phosphorylated at Thr156.

Phospho-AP2M1 (Thr156) Antibody只能检测内源的Thr156位点磷酸化的AP2M1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr156 of human AP2M1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源AP2M1 Thr156位点周围残基相应的磷酸化多肽来免疫动物而获得。抗体通过protein A 和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and either untreated or H2O2-treated (4 mM, 30 minutes), using Phospho-AP2M1 (Thr156) Antibody (upper) or an AP2M1 rabbit mAb (lower).

Western blot 免疫印记。用 Phospho-AP2M1 (Thr156) Antibody (上图) 或者AP2M1 rabbit mAb 兔单抗 (下图)研究血清饥饿过夜,并未经处理或经过H2O2(4mM, 30分钟)处理的HeLa细胞的细胞提取液。

Background

The AP-2 coat assembly protein complex is an important component of clathrin coat pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ and σ protein subunits. The 50 kDa μ subunit (AP-2μ, AP2M1) is located at the core of the AP-2 complex and mediates the interaction between the cargo protein and the clathrin coated pit (1-4). The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif YXXφ found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit. Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6). AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10) and ion channel proteins (11). Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13). Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14).

AP-2包裹组装蛋白复合体是网格蛋白包膜凹陷的重要组分,并参与到受体介导质膜内吞过程(1-3)。每一个AP-2 异源四聚体由 α、β、μ 和σ 蛋白亚单位组成。50 kDa的 μ 亚单元(AP-2μ, AP2M1)定位于AP-2复合体的核心部分,并介导载物蛋白和网格蛋白的凹陷之间的相互作用(1-4)。AP2M1的C-末端区域能够识别基于酪氨酸的内吞分类基序YXXφ,YXXφ是在载物蛋白中发现并能帮助载物蛋白转到网格蛋白的凹陷中。在非常规情况下, 基于酪氨酸的内吞分类信号也能促进载物蛋白和AP2M1之间的互作 (5,6)。AP2M1 在细胞分子信号中发挥了重要作用,因其能够偶联受体介导的内吞,并参与到膜受体(基质金属蛋白酶(10) 和离子通道蛋白(11))介导的信号通路(7-9)。特定的AP2M1位点的磷酸化,并且结合脂类到转化器蛋白,能够调控AP2M1的活性(12,13)。转化器蛋白相关的激酶1 (AAK1) 促使AP2M1蛋白在Thr156位点磷酸化,这能够刺激AP2M1与载物蛋白信号结合的亲和性 (14)。

  1. Kirchhausen, T. (2002) Cell 109, 413-6.
  2. Ohno, H. et al. (1995) Science 269, 1872-5.
  3. Traub, L.M. (2003) J Cell Biol 163, 203-8.
  4. Boll, W. et al. (1996) EMBO J 15, 5789-95.
  5. Royle, S.J. et al. (2002) J Biol Chem 277, 35378-85.
  6. Royle, S.J. et al. (2005) J Cell Sci 118, 3073-80.
  7. Chin, Y.R. and Horwitz, M.S. (2005) J Virol 79, 13606-17.
  8. Wernick, N.L. et al. (2005) J Biol Chem 280, 7309-16.
  9. Johannessen, L.E. et al. (2006) Mol Cell Biol 26, 389-401.
  10. Uekita, T. et al. (2001) J Cell Biol 155, 1345-56.
  11. Chen, Z. et al. (2006) Am J Respir Cell Mol Biol 35, 127-32.
  12. Höning, S. et al. (2005) Mol Cell 18, 519-31.
  13. Olusanya, O. et al. (2001) Curr Biol 11, 896-900.
  14. Conner, S.D. and Schmid, S.L. (2002) J Cell Biol 156, 921-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications