Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841

LIM Kinase  

No. Size Price
3841L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
3841S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3841T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
3841 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 72 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody detects transfected levels of LIMK1 and LIMK2 only when phosphorylated at threonine 508 or 505.

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody仅检测在threonine 508或505位点磷酸化的转染的LIMK1和LIMK2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr508 of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (lane 1), transfected with Wild-type LIMK1 (lanes 2 and 3) or with LIMK1 T508A mutant (lanes 4 and 5), using Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (top), LIMK1 Antibody #3842 (middle) or HA-Tag (262K) mAb #2362 (bottom). Cells were either untreated (lanes 1, 2 and 4) or treated with PMA (lanes 3 and 5). (Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.)

使用Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (上图)、LIMK1 Antibody #3842 (中图)或HA-Tag (262K) mAb #2362 (下图),免疫印迹(Western Blot)分析COS细胞中未转染(第1道)、转染Wild-type LIMK1 (第2、3道)或转染LIMK1 T508A mutant (第4、5道)。细胞分为未处理组(第1、2、4道)和PMA处理(第3、5道)。(Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.)


LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

LIM激酶(LIMK1 and LIMK2)是丝氨酸/苏氨酸激酶,该蛋白在它们的氨基端调节区域含有两个锌指结构其被认为是LIM结构(1)。Rho-family GTPases, PAKs和ROCK的下游的LIM激酶涉及肌动蛋白细胞骨架调节(2,3)。PAKs和ROCK激酶使LIMK1或LIMK2蛋白在激活环里保守的Thr508 或Thr505位点磷酸化,这增加了LIMK活性(3-5)。通过在cofilin蛋白的氨基末端Ser3位点残基磷酸化,活化的LIM 激酶抑制cofilin蛋白的肌动蛋白解聚活性 (6,7)。

  1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
  2. Maekawa, M. et al. (1999) Science 285, 895-898.
  3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
  4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
  5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
  6. Arber, S. et al. (1998) Nature 393, 805-809.
  7. Yang, N. et al. (1998) Nature 393, 809-812.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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