Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841

LIM Kinase  

No. Size Price
3841L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3841S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3841T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3841 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 72 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody detects transfected levels of LIMK1 and LIMK2 only when phosphorylated at threonine 508 or 505.

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody仅检测在threonine 508或505位点磷酸化的转染的LIMK1和LIMK2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr508 of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源LIMK1蛋白Thr508位点周围相应磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (lane 1), transfected with Wild-type LIMK1 (lanes 2 and 3) or with LIMK1 T508A mutant (lanes 4 and 5), using Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (top), LIMK1 Antibody #3842 (middle) or HA-Tag (262K) mAb #2362 (bottom). Cells were either untreated (lanes 1, 2 and 4) or treated with PMA (lanes 3 and 5). (Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.)

使用Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (上图)、LIMK1 Antibody #3842 (中图)或HA-Tag (262K) mAb #2362 (下图),免疫印迹(Western Blot)分析COS细胞中未转染(第1道)、转染Wild-type LIMK1 (第2、3道)或转染LIMK1 T508A mutant (第4、5道)。细胞分为未处理组(第1、2、4道)和PMA处理(第3、5道)。(Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.)

Background

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

LIM激酶(LIMK1 and LIMK2)是丝氨酸/苏氨酸激酶,该蛋白在它们的氨基端调节区域含有两个锌指结构其被认为是LIM结构(1)。Rho-family GTPases, PAKs和ROCK的下游的LIM激酶涉及肌动蛋白细胞骨架调节(2,3)。PAKs和ROCK激酶使LIMK1或LIMK2蛋白在激活环里保守的Thr508 或Thr505位点磷酸化,这增加了LIMK活性(3-5)。通过在cofilin蛋白的氨基末端Ser3位点残基磷酸化,活化的LIM 激酶抑制cofilin蛋白的肌动蛋白解聚活性 (6,7)。

  1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
  2. Maekawa, M. et al. (1999) Science 285, 895-898.
  3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
  4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
  5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
  6. Arber, S. et al. (1998) Nature 393, 805-809.
  7. Yang, N. et al. (1998) Nature 393, 809-812.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications