Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Forskolin #3828

adenylyl cyclase   Coleonol   Colforsin   cyclic AMP   gene transcription   ion transport   protein phosphorylation  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, forskolin-treated (30 µM for 20 min.) or untreated, using Phospho-CREB (Ser133) Antibody #9191 (upper) or CREB Antibody #9192 (lower).对SH-SY5Y细胞抽提液,30 uM forskolin处理20分钟或未处理,使用Phospho-CREB (Ser133)抗体#9191(上图)或CREB抗体#9192(下图)进行Western blot分析。

Directions for Use

Forskolin is soluble in DMSO and methanol. DMSO solutions can be stored at -20ºC in the dark for at least six months.

Forskolin在二甲基亚砜和甲醇中可溶。DMSO溶液可在-20℃避光保存至少六个月。

Background

Forskolin, a naturally occurring diterpene from the Indian plant, Coleus forskohlii, activates adenylate cyclase, and thus increases the intracellular cAMP concentration (1). The second messenger cAMP activates cAMP-dependent protein kinase (PKA or cAPK) and controls many cellular mechanisms such as gene transcription, ion transport and protein phosphorylation (2).

Forskolin是从印度植物毛喉鞘蕊花中自然产生的二萜类物质,可激活腺苷酸环化酶,从而增加细胞内cAMP浓度(1)。第二信使环磷酸腺苷(cAMP)在哺乳动物细胞中激活cAMP依赖的蛋白激酶(PKA或者cAPK),控制如基因转录、离子转运和蛋白磷酸化等许多细胞内机制(2)。

Purity is greater than 99% as determined by HPLC and TLC.

使用HPLC和TLC确定其纯度大于99%。

  1. Laurenza, A. et al. (1989) Trends Pharmacol Sci 10, 442-7.
  2. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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