Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb #3817

kdr   vegf   vegf receptor   vegf receptor 2   vegf receptor-2   vegf receptor2   vegfr   vegfr 2   vegfr-2   vegfr2  

No. Size Price
3817S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3817 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 230 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb only detects endogenous levels of VEGFR2 proteins when phosphorylated at Tyr1059. Since VEGF receptors 1, 2 and 3 share identical sequences within the epitope region, this antibody can also detect VEGF receptors 1 and 3 when phosphorylated at corresponding tyrosine residues.

磷酸化VEGF Receptor 2 (Tyr1059) (D5A6) 兔单抗仅在Tyr1059磷酸化时才能检测内源VEGFR2 蛋白的水平。因为VEGF receptors 1, 2 和3 在表位区具有相通的序列,所以本抗体同样能检测相应酪氨酸位点磷酸化的VEGF receptors 1 和 3 。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1059 of human VEGF receptor 2.

单克隆抗体用合成的磷酸化多肽免疫动物得到,该多肽根据人VEGF receptor 2 的Tyr1059附近的氨基酸合成。

Western Blotting

Western Blotting

Western blot analysis of PAE/CKR cells, untreated or stimulated with CSF-1, using Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb (upper) and VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower). PAE/CKR cells express a chimeric receptor made up of human CSF-1 receptor extracellular domain and mouse VEGF receptor 2 transmembrane and intracellular domains. 用Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb (上)和 VEGF Receptor 2 (55B11) Rabbit mAb #2479 (下)对未经处理的或经过CSF-1刺激的PAE/CKR 细胞进行免疫印迹检测。PAEC/CKR 细胞表达含有人的CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体。


Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

血管内皮生长因子受体2 (VEGFR2, KDR, Flk-1)是内皮细胞上VEGF诱导信号时的一个主要受体。和配体结合之后,VEGFR2发生自磷酸化并被活化(1)。VEGFR2 主要的自磷酸化受体位于激酶插入区 (Tyr951/996)和酪氨酸激酶催化区(Tyr1054/1059) (2)。受体的激活会导致对包括Shc, Grb2, PI3K激酶, Nck 和蛋白酪氨酸磷酸化酶SHP-1 和 SHP-2接头蛋白的快速招募(3)。Tyr1212的磷酸化为Grb2提供了结合位点而且磷酸化的Tyr1175能结合PI3K激酶的p85 亚基以及PLCγ和 Shb (1,4,5)。VEGFR2信号传导对于实现VEGF刺激引起的增殖、趋化和发芽,以及体外培养的内皮细胞的增殖和体内血管的发生是必需的(6-8)。

  1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
  2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
  3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
  4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
  5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
  6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
  7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
  8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

Application References

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Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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