Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb #3806

AVO   AVO3   mAVO3   mTOR   rapamycin   rapamycin-insensitive companion of mTOR (Rictor)   Raptor   rictor   tor  

No. Size Price
3806S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3806T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3806 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 200 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb detects endogenous levels of rictor protein only when phosphorylated at Thr1135.

Phospho-Rictor (Thr1135) (D30A3) 大鼠单克隆抗体能够检测在Thr1135位点磷酸化的内源性的rictor 蛋白含量。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1135 of human rictor protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved HeLa cells, untreated or treated with IGF-1 (50 ng/ml, 30 minutes) and λ phosphatase, using Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb (upper) or Rictor (53A2) Rabbit mAb #2114 (lower).

Western blot a分析血清饥饿的HeLa 细胞提取物,分别为未处理组,IGF-1 (50 ng/ml, 30 minutes) 和λ 磷酸酶处理组,应用Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb兔单抗(上图)或者Rictor (53A2) Rabbit mAb兔单抗 #2114(下图)。


Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity (6-8).

细胞生长是一个基本的生物学过程,细胞积累物质并增加体积。哺乳动物TOR(mTOR)信号通路通过调节生长因子驱动的信号和能量及营养信号调控细胞生长(1)。mTOR是一个有两种不同形式复合物的蛋白激酶。一个复合物包含mTOR,GβL和raptor,是rapamycin的靶点。另一个复合物包括mTOR,GβL,SIN1和rictor,对rapamycin不敏感(1)。mTOR-rictor复合物体外磷酸化修饰Akt/PKB的Ser473位点(2)。磷酸化后可充分激活Akt / PKB。此外,在3T3-L1脂肪细胞里siRNA降低rictor的表达可以抑制Ser473位点磷酸化(3)。这个复合物也可以磷酸化抗rapamycin的S6K1突变体,另一个mTOR效应分子(4)。rictor 蛋白在Thr1135位点磷酸化是CST公司采用PhosphoScan®, CST的LC-MS/MS 平台鉴定发生磷酸化的位点(5)。另外的实验表明rictor在Thr1135位点被p70 S6K磷酸化,负调控mTORC2蛋白复合体,他们又是控制Akt活性的负反馈机制(6-8)。

  1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
  2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
  4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.
  5. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  6. Dibble, C.C. et al. (2009) Mol Cell Biol 29, 5657-70.
  7. Julien, L.A. et al. (2010) Mol Cell Biol 30, 908-21.
  8. Treins, C. et al. (2010) Oncogene 29, 1003-16.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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