Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-HER4/ErbB4 (Tyr984) Antibody #3790

No. Size Price
3790S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3790 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 180 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-HER4/ErbB4 (Tyr984) Antibody detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr984. This antibody may cross-react with other tyrosine-phosphorylated proteins.

磷酸化HER4/ErbB4 (Tyr984) 抗体仅在Tyr984位点磷酸化时能检测到内源HER4/ErbB4蛋白的表达。本抗体可能与其它酪氨酸磷酸化的蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr984 of human HER4/ErbB4. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的HER4/ErbB4蛋白Tyr984附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from T-47D cells, untreated or heregulin-stimulated, using Phospho-HER4/ErbB4 (Tyr984) Antibody (upper) and HER4/ErbB4 (111B2) Rabbit mAb#4795 (lower). Western blot法检测细胞的提取物:未处理或用neuregulin (NRG)处理的T47D细胞。使用抗体为:Phospho-HER4/ErbB4 (Tyr984) Antibody (上) 和HER4/ErbB4 (111B2) Rabbit mAb#4795 (下)。


The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).


  1. Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
  2. Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
  3. Carpenter, G. (2003) Exp Cell Res 284, 66-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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