Cell Signaling Technology

Product Pathways - Innate Immunity

MX1 (D3W7I) Rabbit mAb #37849

MX1   MxA  

No. Size Price
37849S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
37849 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 76 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MX1 (D3W7I) Rabbit mAb recognizes endogenous levels of total MX1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu292 of human MX1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 16 hr; +), using MX1 (D3W7I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of MX1 from extracts of HeLa cells treated with Human Interferon-α1 #8927 (10 ng/ml, 16 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MX1 (D3W7I) Rabbit mAb. Western blot analysis was performed using MX1 (D3W7I) Rabbit mAb.

Background

MX1 (Myxovirus resistance protein 1/MxA) is an interferon-inducible antiviral protein that confers resistance to RNA viruses (1-4). MX1 has GTPase activity, and GTP-bound MX1 adopts a conformation that enables interaction with viral nucleocapsids (5-7). This interaction blocks transport of viral nucleocapsids to the nucleus, which prevents transcription of the viral genome (7,8). Structural studies suggest that the antiviral activity of MX1 involves the formation of MX1 oligomeric rings around viral nucleocapsids (9-12).

  1. Horisberger, M.A. and Hochkeppel, H.K. (1985) J Biol Chem 260, 1730-3.
  2. Staeheli, P. et al. (1985) J Biol Chem 260, 1821-5.
  3. Staeheli, P. and Haller, O. (1985) Mol Cell Biol 5, 2150-3.
  4. Staeheli, P. et al. (1986) Cell 44, 147-58.
  5. Nakayama, M. et al. (1991) J Biol Chem 266, 21404-8.
  6. Kochs, G. and Haller, O. (1999) J Biol Chem 274, 4370-6.
  7. Kochs, G. and Haller, O. (1999) Proc Natl Acad Sci U S A 96, 2082-6.
  8. Kochs, G. et al. (2002) Proc Natl Acad Sci U S A 99, 3153-8.
  9. Kochs, G. et al. (2002) J Biol Chem 277, 14172-6.
  10. Gao, S. et al. (2010) Nature 465, 502-6.
  11. Haller, O. et al. (2010) J Biol Chem 285, 28419-24.
  12. Gao, S. et al. (2011) Immunity 35, 514-25.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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