Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776

No. Size Price
3776S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3776 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 125 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine,

Specificity / Sensitivity

Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb detects endogenous levels of Jak2 when phosphorylated at Tyr1007/1008. This antibody can also detect single phosphorylation at either 1007 or 1008. This antibody may cross-react with phospho-Jak1 and phospho-Tyk2.

Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb兔单抗只能检测内源的在Tyr1007/1008位点磷酸化的Jak2蛋白。此抗体也可检测在Tyr1007 或 Tyr1008位点单磷酸化的Jak2。抗体也可与磷酸化的Jak 和 Tyk2蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Tyr1007/1008 of human Jak2.

此单克隆抗体是通过合成人源对应的Jak2 Tyr1007/1008位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from UT-7 and BaF3 cells, untreated or treated with GM-CSF or IL-3 (5 minutes), using Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776 (top) or total Jak2 (D2E12) XP® Rabbit mAb #3230 (bottom).Western免疫印迹。用Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776 (上图) 或 total Jak2 (D2E12) XP® Rabbit mAb #3230 (下图)研究未经处理的和经GM-CSF 或IL-3 (5 min)处理的UT-7 和BaF3 细胞的细胞提取液。

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Janus家族中的酪氨酸激酶成员(Jak1, Jak2, Jak3和Tyk2)通过配体结合到一些相关的细胞因子受体上而被激活(1)。除了细胞因子受体的激活方式,Jak蛋白能自身磷酸化和磷酸化与其相关的受体从而为信号蛋白提供多个结合位点。这些信号蛋白如Stats (2), Shc (3), 胰岛素受体底物(4) and 成簇黏附激酶 (FAK) (5)都是典型的含有SH2 或者其它的磷酸化酪氨酸结合区域。

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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