Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Jak2 (Tyr1007/1008) Antibody #3771

Janus family tyrosine kinase  

No. Size Price
3771L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3771S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3771 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 125 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat, Bovine,

Specificity / Sensitivity

Phospho-Jak2 (Tyr1007/1008) Antibody detects endogenous levels of Jak2 only when phosphorylated at Tyr1007/1008. This antibody may cross-react with phospho-Jak1 and phospho-Tyk2.

Phospho-Jak2 (Tyr1007/1008) 抗体只能检测内源的在Tyr1007/1008位点磷酸化的Jak2蛋白。抗体也可与磷酸化的Jak1 和 Tyk2蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007/1008 of human Jak2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的Jak2 Tyr1007/1008位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from UT7 cells, untreated or treated with GM-CSF (5 min), and BaF3 cells, untreated or treated with IL-3 (5 min), using Phospho-Jak2 (Tyr1007/1008) Antibody #3771 (upper) or total Jak2 (D2E12) XP® Rabbit mAb #3230 (lower).Western免疫印迹。用Phospho-Jak2 (Tyr1007/1008) Antibody #3771 (上图) 或 total Jak2 (D2E12) XP® Rabbit mAb #3230 (下图)研究未经处理的和经GM-CSF (5 min)处理的UT7细胞的细胞提取液和未经处理的和经IL-3 (5 min)处理的BaF3细胞的细胞提取液 。


Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Janus家族中的酪氨酸激酶成员(Jak1, Jak2, Jak3和Tyk2)通过配体结合到一些相关的细胞因子受体上而被激活(1)。除了细胞因子受体的激活方式,Jak蛋白能自身磷酸化和磷酸化与其相关的受体从而为信号蛋白提供多个结合位点。这些信号蛋白如Stats (2), Shc (3), 胰岛素受体底物(4) and 成簇黏附激酶 (FAK) (5)都是典型的含有SH2 或者其它的磷酸化酪氨酸结合区域。

The tyrosine residues 1007/1008 of Jak2 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).



  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
  6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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