Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-IP3 Receptor (Ser1756) Antibody #3760

No. Size Price
3760S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3760 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 320 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-IP3 Receptor (Ser1756) Antibody detects endogenous levels of IP3 Receptor only when phosphorylated at Ser1756.

Phospho-IP3 Receptor(Ser1756)抗体识别内源性的Ser1756磷酸化的IP3受体。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1756 of rat IP3 receptor. Antibodies are purified by peptide affinity chromatography.

该多克隆抗体用与大鼠IP3受体中Ser1756位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain using Phospho-IP3 Receptor (Ser1756) Antibody. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with IP3 Receptor (Ser1756) phosphopeptide or with IP3 Receptor (Ser1756) non-phosphopeptide prior to incubating the membrane.

对小鼠脑抽提液使用Phospho-IP3 Receptor (Ser1756)抗体进行Western blot分析。磷酸化特异性由无肽段抗体预孵,及孵膜前伴IP3 Receptor (Ser1756)磷酸化或非磷酸化验证。

Background

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. It functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual receptor subunits, resulting in homo- or hetero-oligomerization of the receptor isoform, form a functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release (3,4). IP3R1-mediated Ca2+ release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).

肌醇1,4,5 - 三磷酸受体,也被称为IP3R 或InsP3R,是细胞内钙释放通道家族的成员,位于内质网中。IP3R可作为Ca2 +细胞内储存的钙离子释放通道的功能。有三种IP3受体类型(IP3R1,2,和3),第二信使激活肌醇1,4,5 - 三磷酸(IP3)(1)。四个独立的亚基同源或异源寡聚体化以形成受体的功能通道(2)。IP3R1丝氨酸(1756位)磷酸化,通过环腺苷酸AMP依赖性蛋白激酶(PKA)调节IP3R1至IP3的灵敏性,可能作为Ca2 +释放调节模型(3,4)。IP3R1介导的Ca2 +释放似乎对蒲金耶氏细胞长时程抑制(LTD)的诱导有影响(5)。

  1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
  2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
  3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
  4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
  5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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