Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

JunB (C37F9) Rabbit mAb #3753

AP-1   jun   JunB   sc-73  

No. Size Price
3753S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3753 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 42, 43 Rabbit IgG
IP 1:100
IHC-P 1:800
IF-IC 1:200
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

JunB (C37F9) Rabbit mAb detects endogenous levels of total JunB protein.

JunB (C37F9) Rabbit mAb兔单抗能检测内源性总JunB蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro169 of human JunB.

该单克隆抗体是通过用与人源JunB蛋白Pro169位点周围残基相一致的磷酸化合成肽段免疫兔后获得的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JunB (C37F9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测石蜡包埋的人肺癌组织,使用的抗体为JunB (C37F9) Rabbit mAb。左侧为对照组多肽,右侧为抗原特异性多肽。

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using JunB (C37F9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测: MCF-7细胞,标记的抗体是 JunB (C37F9) Rabbit mAb(绿色)。肌动蛋白丝用Alexa Fluor® 555 鬼笔环肽(红色)标记。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using JunB (C37F9) Rabbit mAb.Western blot检测细胞提取物:各种细胞系,使用的抗体是JunB (C37F9) Rabbit mAb。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of JunB (C37F9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).

JunB蛋白是碱性亮氨酸拉链(bZIP)转录因子,属于Jun家族。该家族包括c-Jun 、JunD蛋白。Jun家族成员与Fos 、ATF蛋白形成同源二聚体或异源二聚体,构成功能性转录因子AP-1(活化蛋白1),它的活性由各种生理和病理刺激调节,如生长因子、传染病和应激性信号(1-4)。由于JunB 蛋白有时会拮抗c-Jun的转录活性,因此该蛋白在小鼠发育过程中可能是Jun蛋白的功能性替代物(5-7)。JunB 蛋白能调节造血干细胞数量,并在慢性粒细胞白血病 (CML)和急性髓细胞性白血病 (AML)的发病机理中发挥重要作用(8,9)。

  1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
  2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
  3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
  4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
  5. Chiu, R. et al. (1989) Cell 59, 979-986.
  6. Schütte, J. et al. (1989) Cell 59, 987-997.
  7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
  8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
  9. Passegué, E. et al. (2004) Cell 119, 431-443.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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