Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-5-Lipoxygenase (Ser663) Antibody #3749

5-LO   5LPG   ALOX5   arachidonate 5-lipoxygenase   LOG5  

No. Size Price
3749S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3749 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 78, 80 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-5-Lipoxygenase (Ser663) Antibody detects overexpressed phospho-5-lipoxygenase protein only when phosphorylated at Ser663.

Phospho-5-Lipoxygenase (Ser663) 抗体能够检测在Ser663位点磷酸化的过表达的5-lipoxygenase蛋白。

Source / Purification

Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser663 of human 5-lipoxygenase protein.

抗体是通过合成人源对应的5-lipoxygenase Ser663位点周围的磷肽段来免疫动物而获得。

Western blot analysis of extracts from COS cells, untransfected, or transfected with either 5-LO wild type or GFP 5-LO (Ser663Ala), using Phospho-5-Lipoxygenase (Ser663) Antibody (upper) and 5-Lipoxygenase (C49G1) Rabbit mAb #3289 (lower). (We are thankful to Dr. Flamand from University of Michigan for providing the overexpression constructs and for his help in characterizing this antibody).Western免疫印迹。用Phospho-5-Lipoxygenase (Ser663) Antibody (上图)和5-Lipoxygenase (49G1) Rabbit mAb #3289(下图)研究未转染和转染了野生型5-LO 或 GFP 5-LO (Ser663Ala)的COS细胞的细胞提取液。(感谢University of Michigan的Flamand博士提供了过表达质粒以及帮助纯化抗体)

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, transfected with 5-LO wild type or GFP 5-LO (Ser663Ala), using Phospho-5-Lipoxygenase (Ser663) Antibody. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide (left), with 5-LO (Ser663) non-phosphopeptide (middle) or 5-LO (Ser663) phosphopeptide (right) prior to incubating the membrane.Western免疫印迹。用Phospho-5-Lipoxygenase (Ser663) Antibody研究转染了野生型5-LO 或GFP 5-LO (Ser663Ala)的COS细胞的细胞提取液。磷酸化特异性抗体是通过在与膜孵育之前先与没有多肽的(左图),未磷酸化5-LO (Ser663)多肽(中间图)或5-LO (Ser663)磷酸化多肽(右图)预培养。

Background

5-Lipoxygenase (5-LO, ALOX5) is an important catalytic enzyme responsible for the biosynthesis of leukotriene LTA4 from arachidonic acid (1,2). Leukotriene synthesis also requires 5-lipoxygenase-activating protein (FLAP, ALOX5AP), a nuclear membrane-bound protein that binds arachidonic acid and is thought to activate 5-LO. A number of related leukotrienes (i.e. B4, C4, D4) are derived from LTA4 and together these lipid mediators function in immune reaction regulation. 5-LO is primarily expressed in polymorphonuclear leukocytes, peripheral blood monocytes, macrophages, and mast cells (1,3). Overexpression of 5-LO protein is seen in certain cancer cells and is associated with poor diagnosis (1,4). Depending upon the cell type, 5-LO is localized to either the cytosol or the nucleus of quiescent cells (5). Following stimulation, 5-LO translocates to the nucleus and associates with FLAP to catalyze LTA4 synthesis (2,3). Phosphorylation of specific residues can regulate 5-LO enzymatic activity. Phosphorylation of 5-LO at Ser523 by PKA family kinases inhibits oxygenase activity (6,7) while MAPKAP2 and ERK family kinase phosphorylation at Ser271 and Ser663 stimulates 5-LO enzymatic activity in vivo (8,9).

5-Lipoxygenase (5-LO, ALOX5)是从花生四烯酸生物合成白细胞三烯LTA4过程中一个重要的催化酶(1,2)。 白细胞三烯合成也需要5-lipoxygenase激活蛋白(FLAP, ALOX5AP), 此蛋白系细胞膜结合蛋白,能够结合花生四烯酸并激活5-LO。一些相关的白细胞三烯 (i.e. B4, C4, D4) 起源于LTA4 和一些在免疫反应调节中发挥作用的脂类介质。5-LO主要在中性粒细胞,外周血单核细胞,巨噬细胞,肥大细胞中表达(1,3)。在一些癌细胞中5-LO过表达并与诊断不利有很大的关系(1,4)。根据不同的细胞种类, 5-LO或者定位在细胞质或者是静止细胞的细胞核(5)。受到刺激后, 5-LO 定位到细胞核中并与FLAP相关催化 LTA4的合成(2,3)。磷酸化特定残基能调控5-LO酶活性。通过PKA家族的激酶磷酸化5-LO的Ser523位点抑制氧化酶的活性(6,7),同时,在体内,MAPKAP2 和ERK家族激酶磷酸化Ser271和Ser663位点能够刺激5-LO酶的活性(8,9)。

  1. Woods, J.W. et al. (1995) J Clin Invest 95, 2035-46.
  2. Evans, J.F. et al. (2008) Trends Pharmacol Sci 29, 72-8.
  3. Radmark, O. et al. (2007) Trends Biochem Sci 32, 332-41.
  4. Chen, X. et al. (2006) Curr Cancer Drug Targets 6, 613-22.
  5. Werz, O. (2002) Curr Drug Targets Inflamm Allergy 1, 23-44.
  6. Luo, M. et al. (2004) J Biol Chem 279, 41512-20.
  7. Luo, M. et al. (2005) J Biol Chem 280, 40609-16.
  8. Werz, O. et al. (2002) FASEB J 16, 1441-3.
  9. Werz, O. et al. (2002) J Biol Chem 277, 14793-800.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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