Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

E2F-1 Antibody #3742

sc-193   sc-251  

No. Size Price
3742S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3742 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 70 Rabbit
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine,

Specificity / Sensitivity

E2F1 Antibody detects endogenous levels of total E2F1 protein. The antibody does not cross-react with other proteins. E2F1 Antibody能够检测内源性E2F1总蛋白。该抗体不与其他蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human E2F1. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对E2F1蛋白的羧基末端的肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with E2F1 siRNA (+) to silence E2F1 protein expression, using E2F-1 Antibody. Western blot方法检测未转染的(-)或转染siRNA(+)以沉默E2F1蛋白表达的HeLa细胞提取物,使用的抗体为E2F-1 Antibody。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Raji cells and either 5 μl of E2F-1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Timeless Intron 1 Primers #7001, human DHFR promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 染色质免疫沉淀方法检测4x106 Raji细胞交联染色质,分别使用5μl E2F-1 Antibody或者2 μl Normal Rabbit IgG #2729,使用的试剂盒为 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集的DNA用real-time PCR技术定量,使用的引物为SimpleChIP® Human Timeless Intron 1 Primers #7001,human DHFR promoter primers,,以及SimpleChIP® Human α Satellite Repeat Primers #4486。以input染色质总量为1,每个样品中IP的DNA量用相对于input 染色质的相对数表示。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with E2F1 siRNA (+) to silence E2F1 protein expression, using E2F-1 Antibody. Western blot方法检测未转染的(-)或转染siRNA(+)以沉默E2F1蛋白表达的HeLa细胞提取物,使用的抗体为E2F-1 Antibody。

Background

The E2F transcription factors are essential for regulation of the cell cycle (1,2). Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit (3, 4). Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control (5-8). E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases (9-11). E2F转录因子对于细胞周期的调节必不可少(1,2)。生理性的E2F是由一个E2F亚基伴随一个DP亚基组成的异源二聚体(3, 4)。E2F家族的六个成员已被鉴定,每一个E2F亚基都有一个DNA结合和二聚化结构域。E2F 1 到5能够激活转录。E2F 1到3能够结合pRb,而E2F-4和E2F-5能够结合p107或者p130,这些相互作用都接受细胞周期的控制(5-8)。E2F-1离体或在体均具有致癌基因的特性。E2F-1能够通过p53依赖或者非依赖的机制诱导凋亡。E2F-1是应激反应性的,并可以被PI3激酶样激酶家族如ATM/ATR激酶调控(9-11)。

  1. Helin, K. (1998) Curr. Opin. Genet. Dev. 8, 28-35.
  2. Dyson, N. (1998) Genes Dev. 12, 2245-2262.
  3. Helin, K. et al. (1993) Genes Dev. 7, 1850-1861.
  4. Wu, C. et al. (1995) Mol. Cell. Biol. 15, 2536-2546.
  5. Takahashi, Y. et al. (2000) Genes Dev. 14, 804-816.
  6. Wu, L. et al. (2001) Nature 414, 457-462.
  7. Gaubatz, S. et al. (2000) Mol. Cell 6, 729-735.
  8. Hurford, R. K. et al. (1997) Genes Dev. 11, 1447-1463.
  9. Tsai, K. Y. et al. (1998) Mol. Cell 2, 293-304.
  10. Garcia, I. et al. (2000) Cell Growth Differ. 11, 91-98.
  11. Lin, W. C. et al. (2001) Genes Dev. 15, 1833-1844.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications