Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SUZ12 (D39F6) XP® Rabbit mAb #3737

CHET9   histone methyltransferase   HMT   JAZF1   JJAZ1   polycomb   zeste  

No. Size Price
3737S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
3737T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
3737 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 83 Rabbit IgG
IP 1:50
IF-IC 1:800
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Pig, Horse,

Specificity / Sensitivity

SUZ12 (D39F6) XP® Rabbit mAb detects endogenous levels of SUZ12 protein.

SUZ12 (D39F6) XP® Rabbit mAb兔单抗能够检测内源性SUZ12蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of the human SUZ12 protein.

通过合成的与人源SUZ12蛋白相应的多肽片段去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP® Rabbit mAb.

使用SUZ12 (D39F6) XP® Rabbit mAb,免疫印迹(Western blot)分析不同细胞中SUZ12 (D39F6)的蛋白水平。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells (left) and NTERA2 cells (right) using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

使用SUZ12 (D39F6) XP® Rabbit mAb (绿色),共聚焦免疫荧光分析HeLa (左图)和NTERA2细胞。DY-554 phalloidin标记微丝蛋白(红色)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

使用SUZ12 (D39F6) XP® Rabbit mAb (绿色),共聚焦免疫荧光分析小鼠胚胎干细胞,生长在mouse embryonic fibroblast (MEF)培养层的细胞。DY-554 phalloidin标记微丝蛋白(红色)。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 5 µl of SUZ12 (D39F6) XP® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HoxA1 intron 1 primers, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,用4 x 106 NCCIT细胞的交联染色质以及5 µl SUZ12 (D39F6) XP® Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用human HoxA1 intron 1 primers、SimpleChIP® Human HoxA2 Promoter Primers #5517和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于总input chromatin的数量的信号(相当于一)。

Background

The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Suppressor of Zeste 12 (SUZ12) is a component of the PRC2 complex, which together with Ezh2 and Eed is absolutely required for histone methyl-transferase activity (6). SUZ12 contains a C2H2 zinc finger domain similar to the zinc finger domains found in sequence-specific DNA binding proteins and may mediate the interaction between EZH2 and nucleosomes (6). SUZ12 is overexpressed in several human tumors, including tumors of the colon, breast and liver (7,8).

polycomb group (PcG)蛋白有助于细胞身份的维持、干细胞自我更新、细胞周期调节和肿瘤形成,这些功能通过维持一些基因的沉默,而这些基因是促进细胞系特异性、细胞死亡和细胞周期停滞(1-4)。PcG蛋白存在两个复合物,它们配合通过表观遗传染色质修饰去维持长期的基因沉默。通过DNA结合转录因子和组蛋白H3在Lys27位点的甲基化,第一个复合物EED-EZH2被招募到基因上。组蛋白H3在Lys27位点的甲基化促进第二个复合物PRC1的招募,其能使组蛋白H2A在Lys119位点泛素化(5)。Zeste 12 (SUZ12)蛋白的抑制剂是PRC2复合物的一个成分,这与Ezh2和Eed一起对于组蛋白甲基转移酶活性是绝对需要的(6)。SUZ12包含一个类似锌指结构域的C2H2锌指结构域,它被发现在序列特异的DNA结合蛋白,并且可能介导在EZH2和核小体之间相互作用(6)。SUZ12在数种人源肿瘤中过表达,包括结肠、乳腺和肝脏的肿瘤(7,8)。

  1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
  2. Lee, T.I. et al. (2006) Cell 125, 301-13.
  3. Cao, R. et al. (2002) Science 298, 1039-43.
  4. Müller, J. et al. (2002) Cell 111, 197-208.
  5. Wang, H. et al. (2004) Nature 431, 873-8.
  6. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
  7. Kirmizis, A. et al. (2003) Mol Cancer Ther 2, 113-21.
  8. Kirmizis, A. et al. (2004) Genes Dev 18, 1592-605.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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