Cell Signaling Technology

Product Pathways - Metabolism

IRP2 (D6E6W) Rabbit mAb #37135

IRP2  

No. Size Price
37135S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
37135 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 105 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

IRP2 (D6E6W) Rabbit mAb recognizes endogenous levels of total IRP2 protein. It does not recognize human IRP1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro731 of human IRP2 protein.

Western blot analysis of extracts from 293 cells, treated overnight with ammonium iron(III) citrate (FAC; 50 μM) or with deferoxamine mesylate (DFO; 50 μM), using IRP2 (D6E6W) Rabbit mAb.

Background

Iron regulatory proteins (IRPs; also known as IREBs) are RNA-binding proteins that recognize iron-responsive elements (IREs) and play an important role in maintaining iron homeostasis in mammalian cells. IREs are conserved cis-regulatory hairpin structures located within the 5’ or 3’ untranslated regions (UTRs) of target mRNAs. IRPs inhibit translation when bound to IREs within the 5’ UTR of mRNA encoding for proteins involved in iron storage, export, and utilization. IRP binding to multiple IREs within the 3’ UTR of transferin receptor 1 (TFR1) mRNA prevents its degradation, thereby augmenting translation of TFR1 and increasing iron uptake into cells (1-3). Dysregulation of IRPs has been associated with human cancers (4-6).

In iron replete cells, FBXL5 targets IRP2 for degradation. Under iron deficiency and/or hypoxic conditions, FBXL5 is destabilized, resulting in IRP2 accumulation and interaction with IRE-containing mRNA (7).

  1. Rouault, T.A. (2006) Nat Chem Biol 2, 406-14.
  2. Wang, J. and Pantopoulos, K. (2011) Biochem J 434, 365-81.
  3. Pantopoulos, K. et al. (2012) Biochemistry 51, 5705-24.
  4. Haro, K.J. et al. (2012) PLoS One 7, e48841.
  5. Wang, W. et al. (2014) Cancer Res 74, 497-507.
  6. Jeong, S.M. et al. (2015) Oncogene 34, 2115-24.
  7. Moroishi, T. et al. (2011) Cell Metab 14, 339-51.

Application References

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