Cell Signaling Technology

Product Pathways - Transcription Factors

GATA-4 (D3A3M) Rabbit mAb #36966

sc-1237   sc-25310  

No. Size Price
36966S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
36966 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 55 Rabbit IgG
F 1:400
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

GATA-4 (D3A3M) Rabbit mAb recognizes endogenous levels of total GATA-4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-4 protein.



Confocal immunofluorescent analysis of Huh7 (left) and HeLa (right) cells using GATA-4 (D3A3M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GATA-4 (D3A3M) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells exhibit very low GATA-4 expression.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (blue) and ATL-16T cells (green) using GATA-4 (D3A3M) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).

GATA-4 is crucial for cardiomyocyte differentiation (4), and not surprisingly, mutations in the GATA-4 gene are implicated in many cardiac diseases, such as tetralogy of Fallot (5), familial and sporadic dilated cardiomyopathy (6,7), and atrial septal defect (8). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (9). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (10). GATA-4 is also a required factor for bone mineralization in osteoblast progenitor cells, where it suppresses TGF-β and activates BMP signaling (11). Research studies have uncovered crosstalk among the transcription factors GATA-4, GATA-6, and KLF5 that promotes gastric cancer development (12). Additional studies describe the role of histone H3K9 hyperacetylation on the GATA-4 promoter in fetal mice that have been exposed to ethanol (13).

  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Gan, L. et al. (2014) Gene Expr Patterns 16, 8-22.
  5. Yang, Y.Q. et al. (2013) Hum Mutat 34, 1662-71.
  6. Li, R.G. et al. (2013) Biochem Biophys Res Commun 439, 591-6.
  7. Li, J. et al. (2014) Gene 548, 174-81.
  8. Mohan, R.A. et al. (2014) Am J Med Genet A 164A, 2732-8.
  9. Walker, E.M. et al. (2014) Dev Biol 392, 283-94.
  10. LaVoie, H.A. (2014) Biol Reprod 91, 38.
  11. Güemes, M. et al. (2014) J Bone Miner Res 29, 2676-87.
  12. Chia, N.Y. et al. (2015) Gut 64, 707-19.
  13. Peng, C. et al. (2014) PLoS One 9, e104135.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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