Cell Signaling Technology

Product Pathways - Metabolism

HMGCS1 (D5W8F) Rabbit mAb #36877

3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase   3-hydroxy-3-methylglutaryl coenzyme A synthase   3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)   HMCS1   HMG-CoA synthase   HMGCS   HMGCS1   Hydroxymethylglutaryl-CoA synthase. cytoplasmic   MGC90332  

No. Size Price
36877S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
36877 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 57 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

HMGCS1 (D5W8F) Rabbit mAb recognizes endogenous levels of total HMGCS1 protein. This antibody does not cross-react with HMGCS2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HMGCS1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HMGCS1 (D5W8F) Rabbit mAb.



Immunoprecipitation of HMGCS1 from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HMGCS1 (D5W8F) Rabbit mAb. Western blot analysis was performed using HMGCS1 (D5W8F) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.


Cytoplasmic 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1) catalyzes the first committed step of mevalonate pathway essential for cholesterol metabolism (1). HMGCS1 transcription is regulated by sterol levels (2). Recent studies showed that miR-223 reduces cholesterol biosynthesis by inhibiting HMGCS1 and methylsterol monooxygenase 1 (3).

  1. Sapir, A. et al. (2014) Proc Natl Acad Sci U S A 111, E3880-9.
  2. Smith, J.R. et al. (1988) J Biol Chem 263, 18480-7.
  3. Vickers, K.C. et al. (2014) Proc Natl Acad Sci U S A 111, 14518-23.

Application References

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