Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TCEB3/Elongin A Antibody #3685

No. Size Price
3685S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
3685 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 100 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TCEB3/Elongin A Antibody recognizes endogenous levels of total TCEB3/Elongin A protein.

TCEB3/Elongin A Antibody能够检测内源性TCEB3/Elongin A总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCEB3/Elongin A protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过合成的人源TCEB3/Elongin A蛋白羧基端相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TCEB3/Elongin A Antibody.

使用TCEB3/Elongin A Antibody,免疫印迹(Western blot)分析不同细胞中TCEB3/Elongin A蛋白水平。


The Elongin complex is a heterotrimer composed of TCEB3/Elongin A, Elongin B (TCEB2), and Elongin C (TCEB1) subunits (1-3). The Elongin complex regulates the rate of RNA polymerase II (RNAPII) transcription elongation by releasing the transient pausing of RNAPII at multiple sites along the DNA. TCEB3/Elongin A is the transcriptionally active subunit, while Elongin B and C subunits play a regulatory role (3,4). TCEB3/Elongin A may be required for expression of a subset of cell cycle regulated genes, and embryonic stem (ES) cells lacking TCEB3/Elongin A show abnormalities in cell size, growth, and cell cycle distribution (5). In addition, the Elongin complex has been shown to interact with the cullin family and RING finger proteins Cul5/Rbx2 upon UV-induced DNA damage, removing arrested RNAPII at sites of DNA damage by ubiquitination and degradation as part of an E3 ubiquitin ligase (6).

Elongin复合物是一个异源三聚体,它是由TCEB3/Elongin A、Elongin B (TCEB2)和Elongin C (TCEB1)亚基组成(1-3)。Elongin复合物调节RNA polymerase II (RNAPII)转录延伸率,这是通过在沿着DNA多位点上释放瞬间暂停。TCEB3/Elongin A是转录激活亚基,而Elongin B和C亚基起到一个调节作用(3,4)。TCEB3/Elongin A可能对于一些细胞周期调节基因的表达是需要的,并且缺少TCEB3/Elongin A蛋白的胚胎干细胞 (embryonic stem,ES)显示在细胞大小、生长和细胞周期分布上不正常(5)。此外,研究显示Elongin复合物与cullin蛋白家族和UV诱导的DNA损伤下RING finger蛋白Cul5/Rbx2相互作用,这通过一个E3泛素连接酶的部分泛素化和降解从而移除了在DNA损伤位点上阻遏的RNAPII蛋白(6)。

  1. Bradsher, J.N. et al. (1993) J Biol Chem 268, 25587-93.
  2. Garrett, K.P. et al. (1994) Proc Natl Acad Sci U S A 91, 5237-41.
  3. Aso, T. et al. (1995) Science 269, 1439-43.
  4. Aso, T. et al. (1996) EMBO J 15, 5557-66.
  5. Yamazaki, K. et al. (2003) J Biol Chem 278, 13585-9.
  6. Yasukawa, T. et al. (2008) EMBO J 27, 3256-66.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :