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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678

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No. Size Price
3678S 100 µl ( 20 western blots ) ¥1,848.00 现货查询 购买询价 防伪查询
3678 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype
W Mouse

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb recognizes the native rabbit IgG. It does not recognize the denatured and reduced rabbit IgG heavy (about 50 kDa) or light (about 25 kDa) chains on western blot.

Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb 识别天然的兔源IgG.在western blot中不能识别兔源IgG变性和还原的重链(50KDa) 的结合或轻链(约25KDa)。

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

单克隆抗体是通过采用天然的全兔IgG免疫动物生产的。

Description

Affinity purified mouse anti-rabbit IgG (Conformation Specific) antibody.

亲和纯化的mouse anti-rabbit IgG (Conformation Specific) antibody。

Western Blotting

Western Blotting

Western blot analysis of decreasing concentrations of total rabbit IgG, reduced and denatured in 1X SDS loading buffer with DTT, using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (Panel B), or Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.Western blot分析浓度递减的全兔IgG,采用带有DTT的SDS上样缓冲液使蛋白还原变性,所用检测抗体为Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (图 A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (图 B), 或 Anti-rabbit IgG, HRP-linked Antibody #7074 (图 C).对于图A和B, 采用Anti-mouse IgG, HRP-linked Antibody #7076可以检测到结合的mouse anti-rabbit IgG mAb ,可以显示出还原变性的兔IgG的位置。

IP

IP

Immunoprecipitation of PRAS40 from untreated HeLa cells using PRAS40 (D23C7) Rabbit mAb #2691 (Lane 2). All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody, including input control in lane 1. Secondary antibodies include Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (Panel B), and Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.免疫沉淀分析未处理组HeLa细胞的PRAS40 ,所用检测抗体为PRAS40 (D23C7) Rabbit mAb #2691 (泳道2)。所由泳道采用PRAS40 (D23C7) Rabbit mAb #2691作为一抗,泳道1中为对照。二抗包括Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mA(图 A) 和 Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 ( 图B) 和Anti-rabbit IgG, HRP-linked Antibody #7074 (图C)。对于图A和B, 采用Anti-mouse IgG, HRP-linked Antibody #7076可以检测到结合的mouse anti-rabbit IgG mAb ,可以显示出还原变性的兔IgG的位置。

Applications

This product has been optimized for use as a secondary antibody in Western blotting applications.

该抗体已经过优化,可以作为二抗用于western blot 实验。

Background

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.

Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb 仅与天然IgG发生反应,不能与变性的和缺少重链和轻链的兔源IgG结合。当免疫沉淀后进行western blotting时,一抗结合的变性的兔源IgG轻链和重链的位置大约分别在25和50KDa,在随后的western blot中,会有相同分子量的条带模糊的蛋白。采用 Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb 减少了这种问题的发生。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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