Cell Signaling Technology

Product Pathways - Metabolism

Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676

acc   acetyl co-a carboxylase   acetyl coa carboxylase  

No. Size Price
3676S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3676T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
3676 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster, Endogenous 280 Rabbit IgG
IHC-P 1:100
F 1:400
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Acetyl-CoA Carboxylase (C83B10) Rabbit mAb detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein.

Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗用于检测内源性所有形式的Acetyl-CoA 羧化酶蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1.

单克隆抗体通过用对应于人源Acetyl-CoA α1蛋白Ser523序列的合成肽免疫动物制备而得

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red).

流式细胞检测293细胞,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗(蓝), 对照为非特异性阴性抗体对照(红)

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

Wetern blot检测多种细胞提取物,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).

激光共聚焦免疫荧光检测NIH/3T3细胞,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗(红),蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084) 。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

免疫组织化学检测石蜡包埋的人乳腺癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

免疫组织化学检测石蜡包埋的人结肠癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

免疫组织化学检测石蜡包埋的人肝脏细胞癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

免疫组织化学检测石蜡包埋的人肺癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).

免疫组织化学检测石蜡包埋的人乳腺癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗,左为对照肽,右为 Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062

Background

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

乙酰辅酶A合成酶(ACC)用于催化脂肪酸合成途径中的关键步骤。分子量为265 kDa的ACCα,是肝脏,脂肪细胞和乳腺中ACC的主要形式,而在骨胳肌和心脏中,其主要形式为280 kDa的ACCβ(1)。ACC可以在Ser79被AMPK磷酸化,或者在Ser1200被PKA磷酸化,这些位点的磷酸化可以抑制ACC的酶活性(2)。ACC也是减肥药物的一个潜在靶点(3,4)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
  6. Fullerton, M.D. et al. (2013) Nat Med 19, 1649-54.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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