Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675

MLC  

No. Size Price
3675L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3675S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3675 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Bovine,Pig, Endogenous 18 Mouse IgG1
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb detects endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. This antibody does not cross-react with the cardiac isoform of myosin light chain 2.

Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb仅检测serine 19位点都磷酸化的内源性myosin light chain 2 (平滑肌)蛋白。该抗体不能与myosin light chain 2的心肌亚型发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosine light chain 2 (smooth muscle).

通过人工合成人源myosin light chain 2(平滑肌)蛋白Ser19位点周围相应的磷酸化片段去免疫动物从而制备出单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293, C2C12, and C6 cells, untreated (-) or lamda phosphatase treated (+), using Myosin Light Chain 2 Antibody #3672 (upper) or Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb (lower).

使用Myosin Light Chain 2 Antibody #3672 (上图)或Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb (下图),免疫印迹(Western Blot)分析HEK293、C2C12和C6细胞中Myosin Light Chain 2和Phospho-Myosin Light Chain 2 (Ser19)蛋白水平,细胞分为untreated (-)或lamda phosphatase treated (+)。

IF-IC

IF-IC

Confocal immunofluorescent images of HeLa cells, untreated (left) or phosphatase-treated (right), labeled with Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb (绿色)标记,共聚焦免疫荧光分析HeLa细胞,细胞分为untreated (左图)或phosphatase-treated (右图)。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084(DNA荧光染料)。

Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).

Myosin蛋白是由六个多肽链组成:两个相同的重链和两对轻链。Myosin light chain 2 (MLC2)又称为myosin regulatory light chain (MRLC or RLC, LC20),它有许多依赖它的分布的亚型。在平滑肌里,MLC2通过myosin light chain kinase (MLCK)以一个Ca2+-钙调蛋白依赖性方式使其在Thr18和Ser19 位点磷酸化(1)。这个磷酸化是与myosin ATPase活性和平滑肌收缩有关(2)。ROCK蛋白也使平滑肌MLC2蛋白Ser19位点磷酸化,这将调节应激纤维的装配(3)。通过PKC激酶使平滑肌MLC2蛋白Ser1/Ser2 and Ser9 位点磷酸化已经被报道抑制myosin ATPase活性 (4,5) 。通过cdc2激酶的磷酸化控制胞质分裂的时间(5)。在心肌亚型上缺乏磷酸化位点的转基因老鼠显示形态和功能的异常(6)。

  1. Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031.
  2. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759.
  3. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
  4. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573.
  5. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
  6. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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