Cell Signaling Technology

Product Pathways - Metabolism

Acetyl-CoA Carboxylase Antibody #3662


No. Size Price
3662S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3662 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Bovine, Endogenous 280 Rabbit
IP 1:50
IHC-P 1:100
F 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Chicken, D. melanogaster, Pig,

Specificity / Sensitivity

Acetyl CoA Carboxylase Antibody detects endogenous levels of all isoforms of acetyl CoA carboxylase protein.

Acetyl CoA Carboxylase Antibody用于检测内源的Acetyl CoA Carboxylase总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys557 of human acetyl CoA carboxylase alpha1. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用对应于人源Acetyl CoA Carboxylase alpha1蛋白Lys557残基序列的合成肽免疫动物制备而得,抗体经蛋白A和肽段亲和层析纯化制得。



Confocal microscopic images of A431 cells showing cytoplasmic stain with Acetyl CoA Carboxylase Antibody (A) compared to an isotype control (B).

A431细胞的激光共聚焦图像,显示胞质被Acetyl CoA Carboxylase Antibody(A)染色,B为阴性对照

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, HeLa, A431, NIH/3T3, L929, C6, H-4-IIE and BAEC cells, using Acetyl CoA Carboxylase Antibody.

Wetern blot检测 293, HeLa, A431, NIH/3T3, L929, C6, H-4-IIE 和BAEC 细胞提取物,所用抗体为Acetyl CoA Carboxylase Antibody。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells, using Acetyl CoA Carboxylase Antibody (blue) compared to a nonspecific negative control antibody (red).

流式细胞检测未处理的Hela细胞系,所用抗体为Acetyl CoA Carboxylase Antibody(蓝), 对照为非特异性阴性抗体(红)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Acetyl CoA Carboxylase Antibody in the presence of control peptide (left) or AcetylCoA Carboxylase Blocking Peptide #1062 (right).

免疫组织化学检测石蜡包埋的人结肠癌组织,所用抗体为Acetyl CoA Carboxylase Antibody,左为对照肽,右为AcetylCoA Carboxylase Blocking Peptide #1062



Immunprecipitation of Acetyl-CoA Carboxylase from AICAR treated C2C12 cell extracts using Acetyl CoA Carboxylase antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.

AICAR处理C2C12细胞提取物免疫沉淀 Acetyl-CoA Carboxylase ,所用抗体为 Acetyl CoA Carboxylase antibody(1道),2道无抗体对照,3道为Input对照


Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

乙酰辅酶A合成酶(ACC)用于催化脂肪酸合成途径中的关键步骤。分子量为265 kDa的ACCα,是肝脏,脂肪细胞和乳腺中ACC的主要形式,而在骨胳肌和心脏中,其主要形式为280 kDa的ACCβ(1)。ACC可以在Ser79被AMPK磷酸化,或者在Ser1200被PKA磷酸化,这些位点的磷酸化可以抑制ACC的酶活性(2)。ACC也是减肥药物的一个潜在靶点(3,4)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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