Cell Signaling Technology

Product Pathways - Adhesion

α-E-Catenin (D9R5E) Rabbit mAb #36611

No. Size Price
36611S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
36611 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 100 Rabbit IgG
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

α-E-Catenin (D9R5E) Rabbit mAb recognizes endogenous levels of total α-E-catenin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala885 of human α-E-Catenin protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-OV-3 (left) and PC-3 (right) cells, using α-E-Catenin (D9R5E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-OV-3 and PC-3 cells using α-E-catenin (D9R5E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-E-catenin (D9R5E) Rabbit mAb.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).

Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).

  1. Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
  2. Yamada, S. et al. (2005) Cell 123, 889-901.
  3. Drees, F. et al. (2005) Cell 123, 903-15.
  4. Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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