Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BLNK (D3P2H) XP® Rabbit mAb #36438

B cell adaptor   B cell linker  

No. Size Price
36438S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
36438T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
36438 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 68, 70 Rabbit IgG
IP 1:50
IHC-P 1:250
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

BLNK (D8P2H) XP® Rabbit mAb recognizes endogenous levels of total BLNK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg282 of human BLNK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BLNK (D3P2H) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). BLNK protein expression is detected in B cell extracts as expected, but is not detected in Jurkat cell extracts.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using BLNK (D3P2H) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using BLNK (D3P2H) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using BLNK (D3P2H) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human whole blood using BLNK (D3P2H) XP® Rabbit mAb co-stained with CD19-APC (left) and CD3-PE (right). CD19+ B cells are positive for BLNK, whereas CD3+ T cells are negative. Analysis was performed on cells in the lymphocyte gate. Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor 488 conjugate) #4412 was used as secondary Ab.

Background

B cell linker protein (BLNK), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts at the interface between BCR-associated Syk and downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino terminus and an SH2 domain at its carboxy terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96, and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules, such as BTK, PLCγ, and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets, such as HPK1, thereby recruiting them to the BCR complex for signaling (5).

  1. Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5.
  2. Fu, C. et al. (1998) Immunity 9, 93-103.
  3. Ishiai, M. et al. (1999) Immunity 10, 117-25.
  4. Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-86.
  5. Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-39.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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