Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Tuberin/TSC2 (28A7) Rabbit mAb #3635

Hamartin   TSC   tuberin   Tuberous sclerosis 2 protein  

No. Size Price
3635S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3635 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 200 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Tuberin/TSC2 (28A7) Rabbit mAb detects endogenous levels of total tuberin.

Tuberin/TSC2 (28A7) Rabbit 单克隆抗体检测内源性的马铃薯球蛋白(Tuberin)的总量。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide from the amino terminus of human tuberin.

单克隆抗体是采用与人类马铃薯蛋白(Tuberin)氨基酸残基相对应的磷酸盐肽免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from control SH-SY5Y, differentiated SH-SY5Y, PC3 and LNCaP cells, using Tuberin/TSC2 (28A7) Rabbit mAb.

应用Tuberin/TSC2 (28A7) Rabbit 单克隆抗体,Western blot 分析SH-SY5Y对照,分化的SH-SY5Y, PC3 和LNCaP等细胞提取物。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Tuberin/TSC2 siRNA I (+) or SignalSilence® Tuberin/TSC2 siRNA II (+), using Tuberin/TSC2 (28A7) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Tuberin/TSC2 (28A7) Rabbit mAb confirms silencing of Tuberin/TSC2 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Tuberin/TSC2 siRNA.

Western blot分析Hela细胞提取物,采用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Tuberin/TSC2 siRNA I (+) 或 SignalSilence® Tuberin/TSC2 siRNA II (+)转染,所用抗体为Tuberin/TSC2 (28A7) Rabbit mAb兔单抗 and α-Tubulin (11H10) Rabbit mAb兔单抗 #2125.Tuberin/TSC2 (28A7) Rabbit mAb兔单抗 确定了Tuberin/TSC2 表达的沉默,α-Tubulin (11H10) Rabbit mAb 作为上样和Tuberin/TSC2 siRNA的特异性的对照。

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

马铃薯球蛋白(Tuberin)是TSC2肿瘤抑制基因的产物,是细胞增殖和肿瘤发展的重要调节因子(1)。TSC2或与之相关的TSC1(hamartin)的突变可以引起结节硬化复合体TSC(tuberous sclerosis complex),是一种常染色体显性遗传病,其特点为发生多种广泛的非恶性肿瘤(2)。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化(3)。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性(3-5)。另外,tuberin可以抑制mTOR(mammalian target of rapamycin), 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白(4E-BP1,一种转录起始抑制因子)的活性,并最终抑制翻译(3,6,7)。

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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