Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

NUT (C52B1) Rabbit mAb #3625

BRD3   BRD3-NUT   BRD4   BRD4-NUT   NMC   nuclear protein in testis   NUT   NUT midline carcinoma   testis nuclear protein  

No. Size Price
3625S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3625 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 150 Rabbit IgG
IP 1:100
IHC-P 1:45
IF-F 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen),

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

NUT (C52B1) Rabbit mAb detects endogenous levels of total NUT protein. The antibody also detects endogenous levels of the BRD4-NUT fusion protein found in NUT midline carcinoma (NMC).

NUT (C52B1) Rabbit mAb兔单抗能够检测内源性NUT总蛋白水平。该抗体在NUT midline carcinoma (NMC)中也能检测内源性BRD4-NUT融合蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the human NUT protein.

通过重组人源NUT蛋白去免疫动物从而制备出此单克隆抗体。

IF-F

IF-F

Confocal immunofluorescent analysis of rat testes using NUT (C52B1) Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

在NUT midline carcinoma (NMC)中 使用NUT (C52B1) Rabbit mAb (绿色)和Pan-Keratin (C11) Mouse mAb #4545 (红色),共聚焦免疫荧光分析大鼠睾丸组织。蓝色= DRAQ5® #4084 (DNA荧光染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human midline carcinoma using NUT (C52B1) Rabbit mAb.

使用NUT (C52B1) Rabbit mAb,免疫组化分析人源中线癌石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analsysis of paraffin-embedded human midline carcinoma using NUT (C52B1) Rabbit mAb.

使用NUT (C52B1) Rabbit mAb,免疫组化分析人源中线癌石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human testes using NUT (C52B1) Rabbit mAb.

使用NUT (C52B1) Rabbit mAb,免疫组化分析人源睾丸组织石蜡切片。

Western Blotting

Western Blotting

Western blot analysis of extracts from rat and human testis using NUT (C52B1) Rabbit mAb.

使用NUT (C52B1) Rabbit mAb,免疫印迹(Western blot)分析大鼠和人源睾丸组织中NUT (C52B1)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from rat testis and NUT midline carcinoma (NMC) cells using NUT (C52B1) Rabbit mAb.

使用NUT (C52B1) Rabbit mAb,免疫印迹(Western blot)分析大鼠睾丸和NUT中线癌(NMC)细胞中NUT (C52B1)蛋白水平。

Background

Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the NUT gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2).

Nuclear protein in testis (NUT)通常局限于睾丸和卵巢的生殖细胞中(1,2)。NUT midline carcinoma (NMC)是一个最近公认的癌症,它通过染色质重排的存在而定义的,这涉及染色体15q14 上的NUT基因(3)。大部分情况下,染色体易位发生在染色体19的NUT和BRD4之间,这导致BRD4-NUT 融合蛋白的形成。在剩余的肿瘤中,不同的NUT重排存在涉及BRD3,它是一个BRD4的紧密的同系物。BRD4-NUT和BRD3-NUT编码融合蛋白,这些蛋白出现通过封闭上皮细胞分化从而有助于致癌。NMCs是侵入性和高度致死癌症,它可以从形态上区分其它弱分化的癌症。假如内源性NUT蛋白限制性表达,则该抗体能通过免疫组化和免疫荧光分析NUT融合蛋白(2)。

  1. French, C.A. et al. (2003) Cancer Res 63, 304-7.
  2. Haack, H. et al. (2009) Am J Surg Pathol 33, 984-91.
  3. French, C.A. et al. (2008) Oncogene 27, 2237-42.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications