Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Tuberin/TSC2 Antibody #3612

sc-893   TSC   tuberin  

No. Size Price
3612S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3612 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 200 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Tuberin/TSC2 Antibody detects endogenous levels of total tuberin protein independent of phosphorylation. This antibody cross-reacts with some other proteins.

Tuberin/TSC2 抗体在不依赖于磷酸化的条件下,检测内源性马铃薯球蛋白总的含量。这个抗体能够和其他蛋白发生 交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human tuberin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml), PDGF and wortmannin-treated (200 nM) or PDGF and rapamycin-treated (5 nM), and MCF-7 cells, untreated or IGF-1 treated for the indicated times, using Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 (upper) or Tuberin/TSC2 Antibody (lower).

Western blot 分析NIH/3T3 细胞提取物,未处理,PDGF处理(50 ng/ml),PDGF 和wortmannin处理(200 nM) 或者PDGF 和rapamycin处理(5 nM),和MCF-7细胞,未处理或者IGF-1处理如图所示的时间点,分别应用Phospho-Tuberin/TSC2 (Thr1462) Antibody #36121(上图)或者Tuberin/TSC2 Antibody (下图)。


Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

马铃薯球蛋白(Tuberin)是TSC2肿瘤抑制基因的产物,是细胞增殖和肿瘤发展的重要调节因子(1)。TSC2或与之相关的TSC1(hamartin)的突变可以引起结节硬化复合体TSC(tuberous sclerosis complex),是一种常染色体显性遗传病,其特点为发生多种广泛的非恶性肿瘤(2)。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化(3)。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性(3-5)。另外,tuberin可以抑制mTOR(mammalian target of rapamycin), 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白(4E-BP1,一种转录起始抑制因子)的活性,并最终抑制翻译(3,6,7)。

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

Application References

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