Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611

sc-293149   TSC2   tuberin  

No. Size Price
3611S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3611 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 200 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-Tuberin/TSC2 (Thr1462) Antibody detects endogenous levels of tuberin only when phosphorylated at threonine 1462. This antibody does not detect tuberin phosphorylated at other sites.

只有在Thr1462位点磷酸化后,Phospho-Tuberin/TSC2 (Thr1462) 抗体检测内源性的马铃薯球蛋白(Tuberin)的含量。 Phospho-Tuberin/TSC2 (Thr1462) 抗体不能检测tuberin的其他磷酸化位点。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用与人类tuberin Thr1462周围相对应的磷酸肽免疫动物生产的。多克隆抗体采用蛋白A和肽亲和色谱法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml), PDGF and wortmannin-treated (200 nM) or PDGF and rapamycin-treated (5 nM), and MCF-7 cells, untreated or IGF-1 treated for the indicated times, using Phospho-Tuberin/TSC2 (Thr1462) Antibody (upper) or Tuberin/TSC2 Antibody #3612 (lower).

Western blot 分析NIH/3T3 细胞提取物,未处理,PDGF处理(50 ng/ml),PDGF 和wortmannin处理(200 nM) 或者PDGF 和rapamycin处理(5 nM),和MCF-7细胞,未处理或者IGF-1处理如图所示的时间点,分别应用Phospho-Tuberin/TSC2 (Thr1462) 抗体(上图)或者Tuberin/TSC2 抗体 #3612 (下图)。

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

马铃薯球蛋白(Tuberin)是TSC2肿瘤抑制基因的产物,是细胞增殖和肿瘤发展的重要调节因子(1)。TSC2或与之相关的TSC1(hamartin)的突变可以引起结节硬化复合体TSC(tuberous sclerosis complex),是一种常染色体显性遗传病,其特点为发生多种广泛的非恶性肿瘤(2)。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化(3)。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性(3-5)。另外,tuberin可以抑制mTOR(mammalian target of rapamycin), 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白(4E-BP1,一种转录起始抑制因子)的活性,并最终抑制翻译(3,6,7)。

Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation, and that the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (3). This data complements Drosophila genetics studies suggesting the possible involvement of the tuberin-hamartin complex in the PI3K/Akt mediated insulin pathway (8-10).马铃薯球蛋白(Tuberin)在PI3K激活后在Ser939 和Thr1462位点被磷酸化,并且人类TSC复合物是PI3K/AKT 信号通路的直接靶向物(3)。这些数据和果蝇( Drosophila)遗传学研究表明tuberin-hamartin 复合物可能在PI3K/AKT 介导的胰岛素通路中取作用(8-10)。

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  8. Gao, X. and Pan, D. (2001) Genes Dev. 15, 1383-1392.
  9. Potter, C. J. et al. (2001) Cell 105, 357-368.
  10. Tapon, N. et al. (2001) Cell 105, 345-355.

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