Cell Signaling Technology

Product Pathways - Development

Notch1 (D1E11) XP® Rabbit mAb #3608

nicd   Notch   sc-6014  

No. Size Price
3608S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
3608 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 120, 300 Rabbit IgG
IP 1:50
IHC-P 1:400
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,

Specificity / Sensitivity

Notch1 (D1E11) XP® Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~300 kDa) and the transmembrane/intracellular region NTM (~120 kDa).

Notch1 (D1E11) XP® Rabbit mAb 兔单抗识别内源性的总Notch1蛋白。它识别Notch1 的全长蛋白(~300 kDa)和跨膜/胞内区域NTM(~120 kDa).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2438 of human Notch1.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Notch1 (D1E11) XP® Rabbit mAb.

使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human inflammatory granulation tissue using Notch1 (D1E11) XP® Rabbit mAb.

使用Notch1 (D1E11) XP® 兔单抗对石蜡包埋的人炎症肉芽组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A2780 (left), Jurkat (center) and RL (right) cell pellets using Notch1 (D1E11) XP® Rabbit mAb. Both A2780 and Jurkat express Notch1, but only Jurkat cells have cleaved Notch1, while RL cells express very low or no Notch1.

使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对石蜡包埋的A2780(左),Jurkat(中)和RL(右)细胞进行免疫组化分析。A2780和Jurkat表达Notch1,但仅有Jurkat表达Notch1的剪切型,而RL细胞中Notch1表达量极低或不表达。

Western Blotting

Western Blotting

Western blot analysis of total cell extract from various cell types using Notch1 (D1E11) XP® Rabbit mAb. The full-length (FL) Notch protein and the cleaved transmembrane/intracellular region (NTM) are indicated.

使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。Notch1的全长(FL)蛋白和剪切后跨膜/细胞内部位(NTM)如图所示。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using Notch1 (D1E11) XP® Rabbit mAb.

使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对石蜡包埋的人结肠组织进行免疫组化分析。

Chromatin IP

Chromatin IP

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM) for 3 days and then either harvested immediately (left panel) or washed and cultured in fresh media for 3 hr (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 5 µl of Notch1 (D1E11) XP® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

CUTLL1细胞在包含γ-分泌酶抑制剂(1 μM) 的培养基中培养三天后,直接收取细胞(左)或清洗后在新鲜培养基中培养3小时。使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 处理的CUTLL1细胞与5μl的Notch1 (D1E11) XP® Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human HES1 Promoter 引物,#7273 human HES4 promoter 引物,和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量相对于input染色质总量进行相对定量,Input中染色质量设定值为1。


Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.

Notch1是一个在细胞发育和命运决定过程中发挥功能的跨膜蛋白(1)。在notch1成熟过程中,notch1分子细胞外结构域会被一个成对碱性氨基酸蛋白酶样的转化酶剪切(2)。在与配体如Delta1结合或是细胞外钙离子耗竭后,notch1的羧基端被释放并随后在Gly1743 和 Val1744之间被切开(3,4)。结果导致细胞内处于活化状态的notch1片段转运至细胞核内激活转录。

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

Application References

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